Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization

L. L. Veeck; J. W.E. Wortham; J. Witmyer; B. A. Sandow; A. A. Acosta; J. E. Garcia; G. S. Jones; H. W. Jones

doi:10.1016/S0015-0282(16)47052-4

Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization

L. L. Veeck, J. W.E. Wortham, J. Witmyer, B. A. Sandow, A. A. Acosta, J. E. Garcia, G. S. Jones, H. W. Jones

Research output: Contribution to journal › Article › peer-review

244 Scopus citations

Abstract

Oocytes of varying stages of maturity were aspirated from follicles primed with either human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG) or a combination of follicle-stimulating hormone (FSH), hMG and hCG. Of the aspirated oocytes from 44 cycles, 74 were considered to be immature by virtue of morphologic characteristics of the oocytes and the degree of intercellular expansion of the associated cumular and membrana granulosa cells. After incubation periods of 22 to 35 hours in a Ham's F-10-based culture medium, these immature oocytes were inseminated with sperm donated by the patient's husband. Ultimately, 44 conceptuses were transferred to the respective uteri of 30 patients. Eight pregnancies were established as a result of these 30 transfers, two of which resulted from the transfer of only developed immature oocytes.

Original language	English (US)
Pages (from-to)	594-602
Number of pages	9
Journal	Unknown Journal
Volume	39
Issue number	5
DOIs	https://doi.org/10.1016/S0015-0282(16)47052-4
State	Published - 1983
Externally published	Yes

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

Access to Document

10.1016/S0015-0282(16)47052-4

Cite this

@article{f119ce9e0c2148ba95b6f83d9fe5da6d,

title = "Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization",

abstract = "Oocytes of varying stages of maturity were aspirated from follicles primed with either human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG) or a combination of follicle-stimulating hormone (FSH), hMG and hCG. Of the aspirated oocytes from 44 cycles, 74 were considered to be immature by virtue of morphologic characteristics of the oocytes and the degree of intercellular expansion of the associated cumular and membrana granulosa cells. After incubation periods of 22 to 35 hours in a Ham's F-10-based culture medium, these immature oocytes were inseminated with sperm donated by the patient's husband. Ultimately, 44 conceptuses were transferred to the respective uteri of 30 patients. Eight pregnancies were established as a result of these 30 transfers, two of which resulted from the transfer of only developed immature oocytes.",

author = "Veeck, {L. L.} and Wortham, {J. W.E.} and J. Witmyer and Sandow, {B. A.} and Acosta, {A. A.} and Garcia, {J. E.} and Jones, {G. S.} and Jones, {H. W.}",

year = "1983",

doi = "10.1016/S0015-0282(16)47052-4",

language = "English (US)",

volume = "39",

pages = "594--602",

journal = "Unknown Journal",

issn = "0309-1708",

publisher = "Elsevier Limited",

number = "5",

}

TY - JOUR

T1 - Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization

AU - Veeck, L. L.

AU - Wortham, J. W.E.

AU - Witmyer, J.

AU - Sandow, B. A.

AU - Acosta, A. A.

AU - Garcia, J. E.

AU - Jones, G. S.

AU - Jones, H. W.

PY - 1983

Y1 - 1983

N2 - Oocytes of varying stages of maturity were aspirated from follicles primed with either human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG) or a combination of follicle-stimulating hormone (FSH), hMG and hCG. Of the aspirated oocytes from 44 cycles, 74 were considered to be immature by virtue of morphologic characteristics of the oocytes and the degree of intercellular expansion of the associated cumular and membrana granulosa cells. After incubation periods of 22 to 35 hours in a Ham's F-10-based culture medium, these immature oocytes were inseminated with sperm donated by the patient's husband. Ultimately, 44 conceptuses were transferred to the respective uteri of 30 patients. Eight pregnancies were established as a result of these 30 transfers, two of which resulted from the transfer of only developed immature oocytes.

AB - Oocytes of varying stages of maturity were aspirated from follicles primed with either human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG) or a combination of follicle-stimulating hormone (FSH), hMG and hCG. Of the aspirated oocytes from 44 cycles, 74 were considered to be immature by virtue of morphologic characteristics of the oocytes and the degree of intercellular expansion of the associated cumular and membrana granulosa cells. After incubation periods of 22 to 35 hours in a Ham's F-10-based culture medium, these immature oocytes were inseminated with sperm donated by the patient's husband. Ultimately, 44 conceptuses were transferred to the respective uteri of 30 patients. Eight pregnancies were established as a result of these 30 transfers, two of which resulted from the transfer of only developed immature oocytes.

UR - http://www.scopus.com/inward/record.url?scp=0020534866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020534866&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(16)47052-4

DO - 10.1016/S0015-0282(16)47052-4

M3 - Article

C2 - 6404659

AN - SCOPUS:0020534866

SN - 0309-1708

VL - 39

SP - 594

EP - 602

JO - Unknown Journal

JF - Unknown Journal

IS - 5

ER -

Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this