Matrix Implanted Laser Desorption Ionization (MILDI) combined with ion mobility-mass spectrometry for bio-surface analysis

A. Tempez; M. Ugarov; T. Egan; J. A. Schultz; A. Novikov; S. Della-Negra; Y. Lebeyec; M. Pautrat; M. Caroff; V. S. Smentkowski; H. Y J Wang; S. N. Jackson; A. S. Woods

doi:10.1021/pr0497879

Matrix Implanted Laser Desorption Ionization (MILDI) combined with ion mobility-mass spectrometry for bio-surface analysis

A. Tempez, M. Ugarov, T. Egan, J. A. Schultz, A. Novikov, S. Della-Negra, Y. Lebeyec, M. Pautrat, M. Caroff, V. S. Smentkowski, H. Y J Wang, S. N. Jackson, A. S. Woods

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The implantation of low velocity massive gold cluster ions allows homogeneous incorporation of a metallic matrix into the near-surface region of rat brain tissues. Subsequent analysis by laser desorption ionization mass spectrometry yields spectra exhibiting molecular ion peaks in the mass range up to 35 kDa similar to those observed by matrix-assisted LDI. Matrix-implanted LDI when combined with ion-mobility preseparation promises to be a useful technique for molecular imaging of biotissues with a laser microprobe.

Original language	English (US)
Pages (from-to)	540-545
Number of pages	6
Journal	Journal of Proteome Research
Volume	4
Issue number	2
DOIs	https://doi.org/10.1021/pr0497879
State	Published - Mar 2005
Externally published	Yes

Keywords

Gold
Ion mobility
Mass spectrometry
Matrix implanted laser desorption ionization
Tissue

ASJC Scopus subject areas

Genetics
Biotechnology
Biochemistry

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10.1021/pr0497879

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Tempez, A., Ugarov, M., Egan, T., Schultz, J. A., Novikov, A., Della-Negra, S., Lebeyec, Y., Pautrat, M., Caroff, M., Smentkowski, V. S., Wang, H. Y. J., Jackson, S. N., & Woods, A. S. (2005). Matrix Implanted Laser Desorption Ionization (MILDI) combined with ion mobility-mass spectrometry for bio-surface analysis. Journal of Proteome Research, 4(2), 540-545. https://doi.org/10.1021/pr0497879

Tempez, A, Ugarov, M, Egan, T, Schultz, JA, Novikov, A, Della-Negra, S, Lebeyec, Y, Pautrat, M, Caroff, M, Smentkowski, VS, Wang, HYJ, Jackson, SN & Woods, AS 2005, 'Matrix Implanted Laser Desorption Ionization (MILDI) combined with ion mobility-mass spectrometry for bio-surface analysis', Journal of Proteome Research, vol. 4, no. 2, pp. 540-545. https://doi.org/10.1021/pr0497879

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abstract = "The implantation of low velocity massive gold cluster ions allows homogeneous incorporation of a metallic matrix into the near-surface region of rat brain tissues. Subsequent analysis by laser desorption ionization mass spectrometry yields spectra exhibiting molecular ion peaks in the mass range up to 35 kDa similar to those observed by matrix-assisted LDI. Matrix-implanted LDI when combined with ion-mobility preseparation promises to be a useful technique for molecular imaging of biotissues with a laser microprobe.",

keywords = "Gold, Ion mobility, Mass spectrometry, Matrix implanted laser desorption ionization, Tissue",

author = "A. Tempez and M. Ugarov and T. Egan and Schultz, {J. A.} and A. Novikov and S. Della-Negra and Y. Lebeyec and M. Pautrat and M. Caroff and Smentkowski, {V. S.} and Wang, {H. Y J} and Jackson, {S. N.} and Woods, {A. S.}",

year = "2005",

month = mar,

doi = "10.1021/pr0497879",

language = "English (US)",

volume = "4",

pages = "540--545",

journal = "Journal of Proteome Research",

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T1 - Matrix Implanted Laser Desorption Ionization (MILDI) combined with ion mobility-mass spectrometry for bio-surface analysis

AU - Tempez, A.

AU - Ugarov, M.

AU - Egan, T.

AU - Schultz, J. A.

AU - Novikov, A.

AU - Della-Negra, S.

AU - Lebeyec, Y.

AU - Pautrat, M.

AU - Caroff, M.

AU - Smentkowski, V. S.

AU - Wang, H. Y J

AU - Jackson, S. N.

AU - Woods, A. S.

PY - 2005/3

Y1 - 2005/3

N2 - The implantation of low velocity massive gold cluster ions allows homogeneous incorporation of a metallic matrix into the near-surface region of rat brain tissues. Subsequent analysis by laser desorption ionization mass spectrometry yields spectra exhibiting molecular ion peaks in the mass range up to 35 kDa similar to those observed by matrix-assisted LDI. Matrix-implanted LDI when combined with ion-mobility preseparation promises to be a useful technique for molecular imaging of biotissues with a laser microprobe.

AB - The implantation of low velocity massive gold cluster ions allows homogeneous incorporation of a metallic matrix into the near-surface region of rat brain tissues. Subsequent analysis by laser desorption ionization mass spectrometry yields spectra exhibiting molecular ion peaks in the mass range up to 35 kDa similar to those observed by matrix-assisted LDI. Matrix-implanted LDI when combined with ion-mobility preseparation promises to be a useful technique for molecular imaging of biotissues with a laser microprobe.

KW - Gold

KW - Ion mobility

KW - Mass spectrometry

KW - Matrix implanted laser desorption ionization

KW - Tissue

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Matrix Implanted Laser Desorption Ionization (MILDI) combined with ion mobility-mass spectrometry for bio-surface analysis

Abstract

Keywords

ASJC Scopus subject areas

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