Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes

Kai Tang; Dongjing Fu; Sven Kötter; Robert J. Cotter; Charles R. Cantor; Hubert Köster

doi:10.1093/nar/23.16.3126

Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes

Kai Tang, Dongjing Fu, Sven Kötter, Robert J. Cotter, Charles R. Cantor, Hubert Köster

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

Matrix-assisted laser desorption/ionizatlon (MALDI) tlme-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidln-coated magnetic beads or controlled pore glass beads). Only the non-Immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxyplcolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.

Original language	English (US)
Pages (from-to)	3126-3131
Number of pages	6
Journal	Nucleic acids research
Volume	23
Issue number	16
DOIs	https://doi.org/10.1093/nar/23.16.3126
State	Published - Aug 25 1995
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes",

abstract = "Matrix-assisted laser desorption/ionizatlon (MALDI) tlme-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidln-coated magnetic beads or controlled pore glass beads). Only the non-Immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxyplcolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.",

author = "Kai Tang and Dongjing Fu and Sven K{\"o}tter and Cotter, {Robert J.} and Cantor, {Charles R.} and Hubert K{\"o}ster",

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AU - Tang, Kai

AU - Fu, Dongjing

AU - Kötter, Sven

AU - Cotter, Robert J.

AU - Cantor, Charles R.

AU - Köster, Hubert

PY - 1995/8/25

Y1 - 1995/8/25

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AB - Matrix-assisted laser desorption/ionizatlon (MALDI) tlme-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidln-coated magnetic beads or controlled pore glass beads). Only the non-Immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxyplcolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.

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JF - Nucleic acids research

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Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes

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