Mass spectral analysis of complex lipids desorbed directly from lyophilized membranes and cells

D. N. Heller; C. Fenselau; R. J. Cotter; P. Demirev; J. K. Olthoff; J. Honovich; M. Uy; T. Tanaka; Y. Kishimoto

doi:10.1016/0006-291X(87)90470-0

Mass spectral analysis of complex lipids desorbed directly from lyophilized membranes and cells

D. N. Heller
, C. Fenselau
, R. J. Cotter
, P. Demirev
, J. K. Olthoff
, J. Honovich
, M. Uy
, T. Tanaka
, Y. Kishimoto

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Three desorption ionization techniques - laser desorption, plasma desorption and fast atom bombardment mass spectrometry - have been applied to lyophilized cells, membranes, lysed cells and various extracts. It has been shown that intact polar lipids are selectively desorbed from biological membranes by these methods and that their mass spectra provide "fingerprints" which reflect the unique biochemical composition of each class of cell or membrane.

Original language	English (US)
Pages (from-to)	194-199
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	142
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(87)90470-0
State	Published - Jan 15 1987
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(87)90470-0

Cite this

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abstract = "Three desorption ionization techniques - laser desorption, plasma desorption and fast atom bombardment mass spectrometry - have been applied to lyophilized cells, membranes, lysed cells and various extracts. It has been shown that intact polar lipids are selectively desorbed from biological membranes by these methods and that their mass spectra provide {"}fingerprints{"} which reflect the unique biochemical composition of each class of cell or membrane.",

author = "Heller, \{D. N.\} and C. Fenselau and Cotter, \{R. J.\} and P. Demirev and Olthoff, \{J. K.\} and J. Honovich and M. Uy and T. Tanaka and Y. Kishimoto",

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doi = "10.1016/0006-291X(87)90470-0",

language = "English (US)",

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T1 - Mass spectral analysis of complex lipids desorbed directly from lyophilized membranes and cells

AU - Heller, D. N.

AU - Fenselau, C.

AU - Cotter, R. J.

AU - Demirev, P.

AU - Olthoff, J. K.

AU - Honovich, J.

AU - Uy, M.

AU - Tanaka, T.

AU - Kishimoto, Y.

PY - 1987/1/15

Y1 - 1987/1/15

N2 - Three desorption ionization techniques - laser desorption, plasma desorption and fast atom bombardment mass spectrometry - have been applied to lyophilized cells, membranes, lysed cells and various extracts. It has been shown that intact polar lipids are selectively desorbed from biological membranes by these methods and that their mass spectra provide "fingerprints" which reflect the unique biochemical composition of each class of cell or membrane.

AB - Three desorption ionization techniques - laser desorption, plasma desorption and fast atom bombardment mass spectrometry - have been applied to lyophilized cells, membranes, lysed cells and various extracts. It has been shown that intact polar lipids are selectively desorbed from biological membranes by these methods and that their mass spectra provide "fingerprints" which reflect the unique biochemical composition of each class of cell or membrane.

UR - https://www.scopus.com/pages/publications/0023116280

UR - https://www.scopus.com/pages/publications/0023116280#tab=citedBy

U2 - 10.1016/0006-291X(87)90470-0

DO - 10.1016/0006-291X(87)90470-0

M3 - Article

C2 - 3545202

AN - SCOPUS:0023116280

SN - 0006-291X

VL - 142

SP - 194

EP - 199

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Mass spectral analysis of complex lipids desorbed directly from lyophilized membranes and cells

Abstract

ASJC Scopus subject areas

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