Lysosomes in human cell cultures. Kinetics of enzyme release from injured particles

L. Gordis; H. M. Nitowsky

doi:10.1016/0014-4827(65)90379-4

Lysosomes in human cell cultures. Kinetics of enzyme release from injured particles

L. Gordis, H. M. Nitowsky

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A subcellular fraction prepared from a heteroploid human cell line had the characteristics of lysosomes in that it contained sedimentable acid hydrolases which could be solubilized by adding Triton X-100. Appearance of soluble acid phosphatase activity during acid incubation of these lysosomes was characterized by an initial rapid rise with no further increase until the third hour of incubation. In addition, sedimentable activity increased, suggesting an alteration of permeability of lysosomes to substrate. Ultraviolet irradiation accelerated the release of acid phosphatase during a 90 min period but was not characterized by the initial rapid rise of soluble activity observed with acid incubation. The effects of acid incubation and ultraviolet irradiation were temperature dependent, with no significant release of enzyme activity at 4 °C. Lysosomes from cells grown with 1.5 × 10^-4 M prednisolone released less acid phosphatase during acid incubation. Those from cells grown in concentrations of steroids as low as 2 × 10^-6 M were similarly affected, but only during the first two hours of acid incubation. Cells grown with 4 × 10^-4 M prednisolone showed inhibition of net protein synthesis and no enhancement of lysosome stability.

Original language	English (US)
Pages (from-to)	556-569
Number of pages	14
Journal	Experimental cell research
Volume	38
Issue number	3
DOIs	https://doi.org/10.1016/0014-4827(65)90379-4
State	Published - Jun 1965

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Cell Biology

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@article{ce280e445db145d19d260e89b6fee7ec,

title = "Lysosomes in human cell cultures. Kinetics of enzyme release from injured particles",

abstract = "A subcellular fraction prepared from a heteroploid human cell line had the characteristics of lysosomes in that it contained sedimentable acid hydrolases which could be solubilized by adding Triton X-100. Appearance of soluble acid phosphatase activity during acid incubation of these lysosomes was characterized by an initial rapid rise with no further increase until the third hour of incubation. In addition, sedimentable activity increased, suggesting an alteration of permeability of lysosomes to substrate. Ultraviolet irradiation accelerated the release of acid phosphatase during a 90 min period but was not characterized by the initial rapid rise of soluble activity observed with acid incubation. The effects of acid incubation and ultraviolet irradiation were temperature dependent, with no significant release of enzyme activity at 4 °C. Lysosomes from cells grown with 1.5 × 10-4 M prednisolone released less acid phosphatase during acid incubation. Those from cells grown in concentrations of steroids as low as 2 × 10-6 M were similarly affected, but only during the first two hours of acid incubation. Cells grown with 4 × 10-4 M prednisolone showed inhibition of net protein synthesis and no enhancement of lysosome stability.",

author = "L. Gordis and Nitowsky, {H. M.}",

note = "Funding Information: 1 This investigation was supported in part by grants from the National Institute of Arthritis and Metabolic Diseases (A-494), the Sational Association for Retarded Children and the Association for Aid of Crippled Children. 2 Surgeon, USPHS, on assignment from the Heart Disease Control Program. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1965",

month = jun,

doi = "10.1016/0014-4827(65)90379-4",

language = "English (US)",

volume = "38",

pages = "556--569",

journal = "Experimental cell research",

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N1 - Funding Information: 1 This investigation was supported in part by grants from the National Institute of Arthritis and Metabolic Diseases (A-494), the Sational Association for Retarded Children and the Association for Aid of Crippled Children. 2 Surgeon, USPHS, on assignment from the Heart Disease Control Program. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1965/6

Y1 - 1965/6

N2 - A subcellular fraction prepared from a heteroploid human cell line had the characteristics of lysosomes in that it contained sedimentable acid hydrolases which could be solubilized by adding Triton X-100. Appearance of soluble acid phosphatase activity during acid incubation of these lysosomes was characterized by an initial rapid rise with no further increase until the third hour of incubation. In addition, sedimentable activity increased, suggesting an alteration of permeability of lysosomes to substrate. Ultraviolet irradiation accelerated the release of acid phosphatase during a 90 min period but was not characterized by the initial rapid rise of soluble activity observed with acid incubation. The effects of acid incubation and ultraviolet irradiation were temperature dependent, with no significant release of enzyme activity at 4 °C. Lysosomes from cells grown with 1.5 × 10-4 M prednisolone released less acid phosphatase during acid incubation. Those from cells grown in concentrations of steroids as low as 2 × 10-6 M were similarly affected, but only during the first two hours of acid incubation. Cells grown with 4 × 10-4 M prednisolone showed inhibition of net protein synthesis and no enhancement of lysosome stability.

AB - A subcellular fraction prepared from a heteroploid human cell line had the characteristics of lysosomes in that it contained sedimentable acid hydrolases which could be solubilized by adding Triton X-100. Appearance of soluble acid phosphatase activity during acid incubation of these lysosomes was characterized by an initial rapid rise with no further increase until the third hour of incubation. In addition, sedimentable activity increased, suggesting an alteration of permeability of lysosomes to substrate. Ultraviolet irradiation accelerated the release of acid phosphatase during a 90 min period but was not characterized by the initial rapid rise of soluble activity observed with acid incubation. The effects of acid incubation and ultraviolet irradiation were temperature dependent, with no significant release of enzyme activity at 4 °C. Lysosomes from cells grown with 1.5 × 10-4 M prednisolone released less acid phosphatase during acid incubation. Those from cells grown in concentrations of steroids as low as 2 × 10-6 M were similarly affected, but only during the first two hours of acid incubation. Cells grown with 4 × 10-4 M prednisolone showed inhibition of net protein synthesis and no enhancement of lysosome stability.

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Lysosomes in human cell cultures. Kinetics of enzyme release from injured particles

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