TY - JOUR
T1 - Lysis of allogeneic human lymphocytes by nonspecifically activated T-like cells
AU - McMichael, A. J.
AU - Gotch, F. M.
AU - Hildreth, J. E K
PY - 1982
Y1 - 1982
N2 - In the generation of cytotoxic effector cells specific for influenza A virus-infected lymphocytes, 3 donors have given an unusual pattern of lytic activity, killing HLA-mismatched target cells. This has been analyzed in detail for 1 donor and 1 of the other 2 shows similar results. Activation only requires culture in medium between 1 and 4 days and parallels development of cell line K562-directed natural killer cells. Target lymphocytes do not need to be virus-infected and appear to be normal lymphocytes. The effector cells carry the surface markers T3 and T8 defined by OKT3/anti-Leu4 and OKT8/anti-Leu2a monoclonal antibodies, respectively. Unlike HLA class 1-restricted or -directed cytotoxic T cells, neither anti-Leu2a nor anti-Leu4 blocked killing in the absence of complement. MHM23, a monoclonal antibody specific for the human lymphocyte function antigen, blocked lysis. The results indicate that these effector cells are related to cytotoxic T lymphocytes, but can lyse allogeneic target cells through a different recognition process. There is some specificity because autologous cells were not killed.
AB - In the generation of cytotoxic effector cells specific for influenza A virus-infected lymphocytes, 3 donors have given an unusual pattern of lytic activity, killing HLA-mismatched target cells. This has been analyzed in detail for 1 donor and 1 of the other 2 shows similar results. Activation only requires culture in medium between 1 and 4 days and parallels development of cell line K562-directed natural killer cells. Target lymphocytes do not need to be virus-infected and appear to be normal lymphocytes. The effector cells carry the surface markers T3 and T8 defined by OKT3/anti-Leu4 and OKT8/anti-Leu2a monoclonal antibodies, respectively. Unlike HLA class 1-restricted or -directed cytotoxic T cells, neither anti-Leu2a nor anti-Leu4 blocked killing in the absence of complement. MHM23, a monoclonal antibody specific for the human lymphocyte function antigen, blocked lysis. The results indicate that these effector cells are related to cytotoxic T lymphocytes, but can lyse allogeneic target cells through a different recognition process. There is some specificity because autologous cells were not killed.
UR - http://www.scopus.com/inward/record.url?scp=0020359661&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020359661&partnerID=8YFLogxK
M3 - Article
C2 - 6984389
AN - SCOPUS:0020359661
SN - 0014-2980
VL - 12
SP - 1002
EP - 1005
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 12
ER -