TY - JOUR
T1 - Lucigenin chemiluminescence and its relationship to mitochondrial respiration in phagocytic cells
AU - Esterline, Russell L.
AU - Trush, Michael A.
PY - 1989/3/15
Y1 - 1989/3/15
N2 - Unstimulated alveolar macrophages, but not polymorphonuclear leukocytes, elicit chemiluminescence from lucigenin which cannot be entirely accounted for by the resting level of superoxide generation. This chemiluminescence was inhibited by both superoxide scavengers and inhibitors of mitochondrial respiration. Although 12-0-tetradecanoyl phorbol-13-acetate addition resulted in a significant increase in cellular superoxide generation, an unexpected decrease in lucigenin chemiluminescence was noted. These results suggest that mitochondria in alveolar macrophages may be a site of lucigenin accumulation and dioxygenation and that 12-0-tetradecanoyl phorbol-13-acetate may modulate this activity.
AB - Unstimulated alveolar macrophages, but not polymorphonuclear leukocytes, elicit chemiluminescence from lucigenin which cannot be entirely accounted for by the resting level of superoxide generation. This chemiluminescence was inhibited by both superoxide scavengers and inhibitors of mitochondrial respiration. Although 12-0-tetradecanoyl phorbol-13-acetate addition resulted in a significant increase in cellular superoxide generation, an unexpected decrease in lucigenin chemiluminescence was noted. These results suggest that mitochondria in alveolar macrophages may be a site of lucigenin accumulation and dioxygenation and that 12-0-tetradecanoyl phorbol-13-acetate may modulate this activity.
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U2 - 10.1016/0006-291X(89)90034-X
DO - 10.1016/0006-291X(89)90034-X
M3 - Article
C2 - 2539116
AN - SCOPUS:0024598916
SN - 0006-291X
VL - 159
SP - 584
EP - 591
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -