TY - JOUR
T1 - LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes
AU - Zhu, Li
AU - Kandasamy, Suresh K.
AU - Liao, Susan E.
AU - Fukunaga, Ryuya
N1 - Funding Information:
We thank Dr. Julius Brennecke (Institute of Molecular Biotechnology, Vienna) for his gifts of fly strains. We thank Dr. Anne Ephrussi (European Molecular Biology Laboratory), Dr. Terry Orr-Weaver (Massachusetts Institute of Technology), Dr. Patrick O’Farrell (University of California, San Francisco), Dr. Paul Macdonald (University of Texas at Austin), Dr. Elmar Wahle (Martin Luther University Halle-Wittenberg), Dr. Christian Lehner (University of Zurich), Dr. Siomi Haruhiko (Keio University), Dr. Akira Nakamura (Kumamoto University), and Dr. Toshie Kai (Osaka University) for their kind gifts of antibodies. We thank Bloomington Drosophila Stock Center, Drosophila Genomics and Genetic Resources, Kyoto Stock Center, and Vienna Drosophila Resource Center for fly strain stocks. We thank the Johns Hopkins University School of Medicine Microscope Facility for use of the Zeiss LSM700, supported by NIH grant S10OD016374 awarded to Dr. Scot C. Kuo. This work was supported by the grants from American Heart Association [15SDG23220028] and the National Institutes of Health [R01GM116841] to R.F.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Post-transcriptional regulation of gene expression plays an essential role during oocyte maturation. Here we report that Drosophila MARF1 (Meiosis Regulator And mRNA Stability Factor 1), which consists of one RNA-recognition motif and six tandem LOTUS domains with unknown molecular function, is essential for oocyte maturation. When tethered to a reporter mRNA, MARF1 post-transcriptionally silences reporter expression by shortening reporter mRNA poly-A tail length and thereby reducing reporter protein level. This activity is mediated by the MARF1 LOTUS domain, which binds the CCR4-NOT deadenylase complex. MARF1 binds cyclin A mRNA and shortens its poly-A tail to reduce Cyclin A protein level during oocyte maturation. This study identifies MARF1 as a regulator in oocyte maturation and defines the conserved LOTUS domain as a post-transcriptional effector domain that recruits CCR4-NOT deadenylase complex to shorten target mRNA poly-A tails and suppress their translation.
AB - Post-transcriptional regulation of gene expression plays an essential role during oocyte maturation. Here we report that Drosophila MARF1 (Meiosis Regulator And mRNA Stability Factor 1), which consists of one RNA-recognition motif and six tandem LOTUS domains with unknown molecular function, is essential for oocyte maturation. When tethered to a reporter mRNA, MARF1 post-transcriptionally silences reporter expression by shortening reporter mRNA poly-A tail length and thereby reducing reporter protein level. This activity is mediated by the MARF1 LOTUS domain, which binds the CCR4-NOT deadenylase complex. MARF1 binds cyclin A mRNA and shortens its poly-A tail to reduce Cyclin A protein level during oocyte maturation. This study identifies MARF1 as a regulator in oocyte maturation and defines the conserved LOTUS domain as a post-transcriptional effector domain that recruits CCR4-NOT deadenylase complex to shorten target mRNA poly-A tails and suppress their translation.
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U2 - 10.1038/s41467-018-06404-w
DO - 10.1038/s41467-018-06404-w
M3 - Article
C2 - 30279526
AN - SCOPUS:85054186405
SN - 2041-1723
VL - 9
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 4031
ER -