Longitudinal Antibody Responses in People Who Inject Drugs Infected with Similar Human Immunodeficiency Virus Strains

Andrew D. Redd, Nicole A. Doria-Rose, Joshua A. Weiner, Martha Nason, Matthew Seivers, Stephen D. Schmidt, Oliver Laeyendecker, Craig Martens, Daniel Bruno, Brandon F. Keele, Nagarajan Raju, Ivelin S. Georgiev, Susanna L. Lamers, Jacquie Astemborski, Gregory D. Kirk, John R. Mascola, Margaret E. Ackerman, Shruti H. Mehta, Thomas C. Quinn

Research output: Contribution to journalArticlepeer-review


Background: Multiple factors influence the human immunodeficiency virus (HIV) antibody response produced during natural infection, leading to responses that can vary in specificity, strength, and breadth. Methods: People who inject drugs identified as recently infected with HIV (n = 23) were analyzed for clustering of their viral sequences (genetic distance, <2%). Longitudinal antibody responses were identified for neutralizing antibody (Nab) potential, and differences in antibody subclass, specificity, and Fc receptor ligation using pseudovirus entry and multiplexed Fc array assays, respectively. Responses were analyzed for differences between subject groups, defined by similarity in the sequence of the infecting virus. Results: Viral sequences from infected individuals were grouped into 3 distinct clusters with 7 unclustered individuals. Subjects in cluster 1 generally had lower antibody response magnitudes, except for antibodies targeting the V1/V2 region. Subjects in clusters 2 and 3 typically had higher antibody response magnitudes, with the Fv specificity of cluster 2 favoring gp140 recognition. NAb responses differed significantly between clusters for 3 of 18 pseudoviruses examined (P <. 05), but there were no differences in overall NAb breadth (P =. 62). Discussion: These data demonstrate that individuals infected with similar viral strains can generate partially similar antibody responses, but these do not drastically differ from those in individuals infected with relatively unrelated strains.

Original languageEnglish (US)
Pages (from-to)756-765
Number of pages10
JournalJournal of Infectious Diseases
Issue number5
StatePublished - Feb 18 2020


  • HIV
  • antibody development
  • cluster linkage
  • neutralizing antibody
  • people who inject drugs

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases


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