Long-term suppression of ocular neovascularization by intraocular injection of biodegradable polymeric particles containing aserpin-derived peptide

Ron B. Shmueli, Masayuki Ohnaka, Akiko Miki, Niranjan B. Pandey, Raquel Lima e Silva, Jacob E. Koskimaki, Jayoung Kim, Aleksander S. Popel, Peter A. Campochiaro, Jordan J. Green

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Aberrant angiogenesis can cause or contribute to a number of diseases such as neovascular age-related macular degeneration (NVAMD). While current NVAMD treatments target angiogenesis, these treatments are not effective for all patients and also require frequent intravitreal injections. New agents and delivery systems to treat NVAMD could be beneficial to many patients. We have recently developed a serpin-derived peptide as an anti-angiogenic agent. Here, this peptide is investigated for activity in human retinal endothelial cells invitro and for reducing angiogenesis in a laser-induced choroidal neovascularization mouse model of NVAMD invivo. While frequent intravitreal injections can be tolerated clinically, reducing the number of injections can improve patient compliance, safety, and outcomes. To achieve this goal, and to maximize the invivo activity of injected peptide, we have developed biodegradable polymers and controlled release particle formulations to extend anti-angiogenic therapy. To create these devices, the anionic peptides are first self-assembled into nanoparticles using a biodegradable cationic polymer and then as a second step, these nanoparticles are encapsulated into biodegradable poly(lactic-co-glycolic acid) (PLGA) microparticles. In situ, these particles show approximately zero-order, linear release of the anionic peptide over 200 days. These particles are made of safe, hydrolytically degradable polymers and have low endotoxin. Long-term invivo experiments in the laser-induced neovascularization model for NVAMD show that these peptide-releasing particles decrease angiogenesis for at least fourteen weeks invivo following a single particle dose and therefore are a promising treatment strategy for NVAMD.

Original languageEnglish (US)
Pages (from-to)7544-7551
Number of pages8
Issue number30
StatePublished - Oct 2013


  • Angiogenesis
  • Controlled drug release
  • Drug delivery
  • Microsphere
  • Ophthalmology
  • Peptide

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Ceramics and Composites
  • Biomaterials
  • Mechanics of Materials


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