TY - JOUR
T1 - Localization of histidine decarboxylase mRNA in rat brain
AU - Bayliss, Douglas A.
AU - Wang, Yan Min
AU - Zahnow, Cynthia A.
AU - Joseph, David R.
AU - Millhorn, David E.
N1 - Funding Information:
The authors express their appreciation to Luisa E. Klingler and D. A. Fenstermacher for their excellent technical assistance. They also thank K. B. Seroogy for his helpful comments throughout the studies. Work from the authors’ laboratories was supported by HL33831, AHA 881108 (D.E.M.), and HD21744 (D.R.J.). D.A.B. is supported by postgraduate scholarship from NSERC, Canada, and by a fellowship from Glaxo Pharmaceutical Co., Research Triangle Park, North Carolina. D.E.M. is a Career Investigator of the American Lung Association.
PY - 1990/8
Y1 - 1990/8
N2 - The recent cloning of a cDNA encoding fetal rat liver histidine decarboxylase (HDC), the synthesizing enzyme for histamine, allows the study of the central histaminergic system at the molecular level. To this end, Northern blot and in situ hybridization analyses were used to determine the regional and cellular distribution of neurons which express HDC mRNA in rat brain. Three hybridizing species which migrate as 1.6-, 2.6-, and 3.5-kb RNA were identified with Northern blots. The major (2.6 kb) and minor (3.5 kb) species, characteristic of HDC mRNA in fetal liver, were expressed at high levels in diencephalon and at just detectable levels in hippocampus, but not in other brain regions. In contrast, the 1.6-kb species was present in all brain regions examined except the olfactory bulb. Cells which contain HDC mRNA were found by in situ hybridization in the hypothalamus; HDC mRNA-containing cells were not detected in other areas, including the hippocampus. Hypothalamic neurons which express HDC mRNA were localized to all aspects of the tuberomammillary nucleus, a result consistent with previous immunohistochemical findings.
AB - The recent cloning of a cDNA encoding fetal rat liver histidine decarboxylase (HDC), the synthesizing enzyme for histamine, allows the study of the central histaminergic system at the molecular level. To this end, Northern blot and in situ hybridization analyses were used to determine the regional and cellular distribution of neurons which express HDC mRNA in rat brain. Three hybridizing species which migrate as 1.6-, 2.6-, and 3.5-kb RNA were identified with Northern blots. The major (2.6 kb) and minor (3.5 kb) species, characteristic of HDC mRNA in fetal liver, were expressed at high levels in diencephalon and at just detectable levels in hippocampus, but not in other brain regions. In contrast, the 1.6-kb species was present in all brain regions examined except the olfactory bulb. Cells which contain HDC mRNA were found by in situ hybridization in the hypothalamus; HDC mRNA-containing cells were not detected in other areas, including the hippocampus. Hypothalamic neurons which express HDC mRNA were localized to all aspects of the tuberomammillary nucleus, a result consistent with previous immunohistochemical findings.
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U2 - 10.1016/1044-7431(90)90036-4
DO - 10.1016/1044-7431(90)90036-4
M3 - Article
AN - SCOPUS:0002152104
SN - 1044-7431
VL - 1
SP - 3
EP - 9
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 1
ER -