Localization of a human gene homologous to the PrP gene on the p ARM of chromosome 20 and detection of PrP-related antigens in normal human brain

Nikolaos K. Robakis; Evelyn A. Devine-Gage; Edmund C. Jenkins; Richard J. Kascsak; W. Ted Brown; Michael S. Krawczun; Wayne P. Silverman

doi:10.1016/0006-291X(86)90796-5

Localization of a human gene homologous to the PrP gene on the p ARM of chromosome 20 and detection of PrP-related antigens in normal human brain

Nikolaos K. Robakis
, Evelyn A. Devine-Gage
, Edmund C. Jenkins
, Richard J. Kascsak
, W. Ted Brown
, Michael S. Krawczun
, Wayne P. Silverman

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

Infectious fractions prepared from scrapie-infected hamster brains contain a protein, PrP 27-30, which shares antigenic determinants with polypeptides found in similarly prepared fractions from patients with Creutzfeldt-Jakob disease. cDNA sequences encoding the hamster PrP 27-30 identified homologous sequences in the human genome as well as in normal human brain mRNA preparations. Antibodies raised against the mouse PrP's identified antigenically related peptides in both normal hamster and human brain as well as in scrapie-infected hamster brain and CJD-affected human brain. By using in situ hybridization we localized the homologous human genomic sequences on the short arm of chromosome 20. Our results indicate that the reportedly unique proteins detected in human CJD preparations derive from normal human gene products.

Original language	English (US)
Pages (from-to)	758-765
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	140
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(86)90796-5
State	Published - Oct 30 1986
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(86)90796-5

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Robakis, N. K., Devine-Gage, E. A., Jenkins, E. C., Kascsak, R. J., Brown, W. T., Krawczun, M. S., & Silverman, W. P. (1986). Localization of a human gene homologous to the PrP gene on the p ARM of chromosome 20 and detection of PrP-related antigens in normal human brain. Biochemical and Biophysical Research Communications, 140(2), 758-765. https://doi.org/10.1016/0006-291X(86)90796-5

Localization of a human gene homologous to the PrP gene on the p ARM of chromosome 20 and detection of PrP-related antigens in normal human brain. / Robakis, Nikolaos K.; Devine-Gage, Evelyn A.; Jenkins, Edmund C. et al.
In: Biochemical and Biophysical Research Communications, Vol. 140, No. 2, 30.10.1986, p. 758-765.

Research output: Contribution to journal › Article › peer-review

Robakis, NK, Devine-Gage, EA, Jenkins, EC, Kascsak, RJ, Brown, WT, Krawczun, MS & Silverman, WP 1986, 'Localization of a human gene homologous to the PrP gene on the p ARM of chromosome 20 and detection of PrP-related antigens in normal human brain', Biochemical and Biophysical Research Communications, vol. 140, no. 2, pp. 758-765. https://doi.org/10.1016/0006-291X(86)90796-5

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title = "Localization of a human gene homologous to the PrP gene on the p ARM of chromosome 20 and detection of PrP-related antigens in normal human brain",

abstract = "Infectious fractions prepared from scrapie-infected hamster brains contain a protein, PrP 27-30, which shares antigenic determinants with polypeptides found in similarly prepared fractions from patients with Creutzfeldt-Jakob disease. cDNA sequences encoding the hamster PrP 27-30 identified homologous sequences in the human genome as well as in normal human brain mRNA preparations. Antibodies raised against the mouse PrP's identified antigenically related peptides in both normal hamster and human brain as well as in scrapie-infected hamster brain and CJD-affected human brain. By using in situ hybridization we localized the homologous human genomic sequences on the short arm of chromosome 20. Our results indicate that the reportedly unique proteins detected in human CJD preparations derive from normal human gene products.",

author = "Robakis, \{Nikolaos K.\} and Devine-Gage, \{Evelyn A.\} and Jenkins, \{Edmund C.\} and Kascsak, \{Richard J.\} and Brown, \{W. Ted\} and Krawczun, \{Michael S.\} and Silverman, \{Wayne P.\}",

note = "Funding Information: ACKNWLEDGEMENTS: We thank Drs. R. Carp and R. Rubenstein discussions, R.G. Weed and M.E. Nascimento for assistance manuscript figures, and G.E. Houck, Jr., S.L. Nolin and P.R. technical assistance. This work was partially supported by NM EAD-G is a fellow of the National Down Syndrome Society. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1986",

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doi = "10.1016/0006-291X(86)90796-5",

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AU - Robakis, Nikolaos K.

AU - Devine-Gage, Evelyn A.

AU - Jenkins, Edmund C.

AU - Kascsak, Richard J.

AU - Brown, W. Ted

AU - Krawczun, Michael S.

AU - Silverman, Wayne P.

N1 - Funding Information: ACKNWLEDGEMENTS: We thank Drs. R. Carp and R. Rubenstein discussions, R.G. Weed and M.E. Nascimento for assistance manuscript figures, and G.E. Houck, Jr., S.L. Nolin and P.R. technical assistance. This work was partially supported by NM EAD-G is a fellow of the National Down Syndrome Society. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1986/10/30

Y1 - 1986/10/30

N2 - Infectious fractions prepared from scrapie-infected hamster brains contain a protein, PrP 27-30, which shares antigenic determinants with polypeptides found in similarly prepared fractions from patients with Creutzfeldt-Jakob disease. cDNA sequences encoding the hamster PrP 27-30 identified homologous sequences in the human genome as well as in normal human brain mRNA preparations. Antibodies raised against the mouse PrP's identified antigenically related peptides in both normal hamster and human brain as well as in scrapie-infected hamster brain and CJD-affected human brain. By using in situ hybridization we localized the homologous human genomic sequences on the short arm of chromosome 20. Our results indicate that the reportedly unique proteins detected in human CJD preparations derive from normal human gene products.

AB - Infectious fractions prepared from scrapie-infected hamster brains contain a protein, PrP 27-30, which shares antigenic determinants with polypeptides found in similarly prepared fractions from patients with Creutzfeldt-Jakob disease. cDNA sequences encoding the hamster PrP 27-30 identified homologous sequences in the human genome as well as in normal human brain mRNA preparations. Antibodies raised against the mouse PrP's identified antigenically related peptides in both normal hamster and human brain as well as in scrapie-infected hamster brain and CJD-affected human brain. By using in situ hybridization we localized the homologous human genomic sequences on the short arm of chromosome 20. Our results indicate that the reportedly unique proteins detected in human CJD preparations derive from normal human gene products.

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Localization of a human gene homologous to the PrP gene on the p ARM of chromosome 20 and detection of PrP-related antigens in normal human brain

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