Localization and physical mapping of the prostate-specific membrane antigen (PSM) gene to human chromosome 11+

Carrie W. Rinker-Schaeffer; Anita L. Hawkins; Sai L. Su; Ron S. Israeli; Constance A. Griffin; John T. Isaacs; Warren D.W. Heston

doi:10.1006/geno.1995.0019

Localization and physical mapping of the prostate-specific membrane antigen (PSM) gene to human chromosome 11+

Carrie W. Rinker-Schaeffer, Anita L. Hawkins, Sai L. Su, Ron S. Israeli, Constance A. Griffin, John T. Isaacs, Warren D.W. Heston

School of Medicine

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The prostate-specific membrane antigen (PSM) was identified by the monoclonal antibody 7E11-C5.3, which was raised against the human prostatic carcinoma cell line LNCaP (3). The PSM antigen is expressed by normal, neoplastic, and metastatic prostatic tissues. The 2.65-kb cDNA encoding the 100-kDa PSM glycoprotein was cloned from LNCaP cells (4). Studies have shown that the expression of PSM is tissue-specific (5). In the present study monochromosomal somatic cell hybrids were used to localize the PSM gene to human chromosome 11. Using this information, initial mapping studies identified two potential PSM gene loci at 11p11.1-p13 and 11q14. Further high-stringency analysis using cosmid probes identified the 11q14 region as the location of the PSM gene.

Original language	English (US)
Pages (from-to)	105-108
Number of pages	4
Journal	Genomics
Volume	30
Issue number	1
DOIs	https://doi.org/10.1006/geno.1995.0019
State	Published - Nov 1995

ASJC Scopus subject areas

Genetics

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@article{f861c70f698b413ba5a1821e1034c0c9,

title = "Localization and physical mapping of the prostate-specific membrane antigen (PSM) gene to human chromosome 11+",

abstract = "The prostate-specific membrane antigen (PSM) was identified by the monoclonal antibody 7E11-C5.3, which was raised against the human prostatic carcinoma cell line LNCaP (3). The PSM antigen is expressed by normal, neoplastic, and metastatic prostatic tissues. The 2.65-kb cDNA encoding the 100-kDa PSM glycoprotein was cloned from LNCaP cells (4). Studies have shown that the expression of PSM is tissue-specific (5). In the present study monochromosomal somatic cell hybrids were used to localize the PSM gene to human chromosome 11. Using this information, initial mapping studies identified two potential PSM gene loci at 11p11.1-p13 and 11q14. Further high-stringency analysis using cosmid probes identified the 11q14 region as the location of the PSM gene.",

author = "Rinker-Schaeffer, {Carrie W.} and Hawkins, {Anita L.} and Su, {Sai L.} and Israeli, {Ron S.} and Griffin, {Constance A.} and Isaacs, {John T.} and Heston, {Warren D.W.}",

note = "Funding Information: This work was supported by NIH Grant CA 5836 DK/CA 47650 and Training Grant CA-09501.",

year = "1995",

month = nov,

doi = "10.1006/geno.1995.0019",

language = "English (US)",

volume = "30",

pages = "105--108",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

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T1 - Localization and physical mapping of the prostate-specific membrane antigen (PSM) gene to human chromosome 11+

AU - Rinker-Schaeffer, Carrie W.

AU - Hawkins, Anita L.

AU - Su, Sai L.

AU - Israeli, Ron S.

AU - Griffin, Constance A.

AU - Isaacs, John T.

AU - Heston, Warren D.W.

N1 - Funding Information: This work was supported by NIH Grant CA 5836 DK/CA 47650 and Training Grant CA-09501.

PY - 1995/11

Y1 - 1995/11

N2 - The prostate-specific membrane antigen (PSM) was identified by the monoclonal antibody 7E11-C5.3, which was raised against the human prostatic carcinoma cell line LNCaP (3). The PSM antigen is expressed by normal, neoplastic, and metastatic prostatic tissues. The 2.65-kb cDNA encoding the 100-kDa PSM glycoprotein was cloned from LNCaP cells (4). Studies have shown that the expression of PSM is tissue-specific (5). In the present study monochromosomal somatic cell hybrids were used to localize the PSM gene to human chromosome 11. Using this information, initial mapping studies identified two potential PSM gene loci at 11p11.1-p13 and 11q14. Further high-stringency analysis using cosmid probes identified the 11q14 region as the location of the PSM gene.

AB - The prostate-specific membrane antigen (PSM) was identified by the monoclonal antibody 7E11-C5.3, which was raised against the human prostatic carcinoma cell line LNCaP (3). The PSM antigen is expressed by normal, neoplastic, and metastatic prostatic tissues. The 2.65-kb cDNA encoding the 100-kDa PSM glycoprotein was cloned from LNCaP cells (4). Studies have shown that the expression of PSM is tissue-specific (5). In the present study monochromosomal somatic cell hybrids were used to localize the PSM gene to human chromosome 11. Using this information, initial mapping studies identified two potential PSM gene loci at 11p11.1-p13 and 11q14. Further high-stringency analysis using cosmid probes identified the 11q14 region as the location of the PSM gene.

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Localization and physical mapping of the prostate-specific membrane antigen (PSM) gene to human chromosome 11+

Abstract

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