@article{7ea93c19ee984f219574e715602633d0,
title = "Local Cues Establish and Maintain Region-Specific Phenotypes of Basal Ganglia Microglia",
abstract = "Microglia play critical roles in tissue homeostasis and can also modulate neuronal function and synaptic connectivity. In contrast to astrocytes and oligodendrocytes, which arise from multiple progenitor pools, microglia arise from yolk sac progenitors and are widely considered to be equivalent throughout the CNS. However, little is known about basic properties of deep brain microglia, such as those within the basal ganglia (BG). Here, we show that microglial anatomical features, lysosome content, membrane properties, and transcriptomes differ significantly across BG nuclei. Region-specific phenotypes of BG microglia emerged during the second postnatal week and were re-established following genetic or pharmacological microglial ablation and repopulation in the adult, indicating that local cues play an ongoing role in shaping microglial diversity. These findings demonstrate that microglia in the healthy brain exhibit a spectrum of distinct functional states and provide a critical foundation for defining microglial contributions to BG circuit function. De Biase et al. show that microglia in different basal ganglia nuclei exhibit region-specific phenotypes, with distinct anatomical features, lysosome content, membrane properties, and transcriptomes. Local regulatory cues guide establishment and maintenance of this regional specialization.",
keywords = "RNA sequencing, density, development, electrophysiology, heterogeneity, microglia, morphology, nucleus accumbens, substantia nigra, ventral tegmental area",
author = "{De Biase}, {Lindsay M.} and Schuebel, {Kornel E.} and Fusfeld, {Zachary H.} and Kamwing Jair and Hawes, {Isobel A.} and Raffaello Cimbro and Zhang, {Hai Ying} and Liu, {Qing Rong} and Hui Shen and Xi, {Zheng Xiong} and David Goldman and Antonello Bonci",
note = "Funding Information: This research was supported by the Intramural Research Program of NIDA and NIAAA. The authors thank D.E. Bergles (Johns Hopkins University) for antibodies to NG2, NIDA IRP Transgenic breeding staff for assistance with animal care, Johns Hopkins Bayview Immunomics Core (supported by NIAMS P30 AR-070254) for FACS services, NIDA IRP Histology Core for use of Imaris software, M. Zemen, P. Gopal, S.P. Ribeiro, S. Scognamiglio, and S. Deshpande for contributions to experiments, J.L. Cadet and C. Brannock for advice in RNA-seq analysis, A. De Biase for assistance in PCR primer design and analysis, and B.K. Harvey, R.M. McDevitt, G. Seabold, and W. Xin for advice and editorial suggestions during manuscript preparation. Funding Information: This research was supported by the Intramural Research Program of NIDA and NIAAA . The authors thank D.E. Bergles (Johns Hopkins University) for antibodies to NG2, NIDA IRP Transgenic breeding staff for assistance with animal care, Johns Hopkins Bayview Immunomics Core (supported by NIAMS P30 AR-070254 ) for FACS services, NIDA IRP Histology Core for use of Imaris software, M. Zemen, P. Gopal, S.P. Ribeiro, S. Scognamiglio, and S. Deshpande for contributions to experiments, J.L. Cadet and C. Brannock for advice in RNA-seq analysis, A. De Biase for assistance in PCR primer design and analysis, and B.K. Harvey, R.M. McDevitt, G. Seabold, and W. Xin for advice and editorial suggestions during manuscript preparation. Publisher Copyright: {\textcopyright} 2017",
year = "2017",
month = jul,
day = "19",
doi = "10.1016/j.neuron.2017.06.020",
language = "English (US)",
volume = "95",
pages = "341--356.e6",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "2",
}