LNCIB human full-length cDNAs collection: Towards a better comprehension of the human transcriptome

Emiliano Dalla; Roberto Verardo; Dejan Lazarević; Luigi Marchionni; James F. Reid; Nabil Bahar; Enio Klarić; Giacomo Marcuzzi; Riccardo Marzio; Anna Belgrano; Danilo Licastro; Claudio Schneider

doi:10.1016/j.crvi.2003.09.029

LNCIB human full-length cDNAs collection: Towards a better comprehension of the human transcriptome

Emiliano Dalla, Roberto Verardo, Dejan Lazarević, Luigi Marchionni, James F. Reid, Nabil Bahar, Enio Klarić, Giacomo Marcuzzi, Riccardo Marzio, Anna Belgrano, Danilo Licastro, Claudio Schneider

Research output: Contribution to journal › Article › peer-review

Abstract

LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23 000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

Original language	English (US)
Pages (from-to)	967-970
Number of pages	4
Journal	Comptes Rendus - Biologies
Volume	326
Issue number	10-11
DOIs	https://doi.org/10.1016/j.crvi.2003.09.029
State	Published - 2003
Externally published	Yes

Keywords

Full-length cDNA
Functional analysis
Human transcriptome

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Agricultural and Biological Sciences(all)

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10.1016/j.crvi.2003.09.029

Cite this

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title = "LNCIB human full-length cDNAs collection: Towards a better comprehension of the human transcriptome",

abstract = "LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23 000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.",

keywords = "Full-length cDNA, Functional analysis, Human transcriptome",

author = "Emiliano Dalla and Roberto Verardo and Dejan Lazarevi{\'c} and Luigi Marchionni and Reid, {James F.} and Nabil Bahar and Enio Klari{\'c} and Giacomo Marcuzzi and Riccardo Marzio and Anna Belgrano and Danilo Licastro and Claudio Schneider",

note = "Funding Information: This study was supported by NCI project UOICA 80200 and CNR project Functional Genomics – Bioinfomatics and Comparative Functional Genomics.",

year = "2003",

doi = "10.1016/j.crvi.2003.09.029",

language = "English (US)",

volume = "326",

pages = "967--970",

journal = "Comptes Rendus - Biologies",

issn = "1631-0691",

publisher = "Elsevier Masson",

number = "10-11",

}

TY - JOUR

T1 - LNCIB human full-length cDNAs collection

T2 - Towards a better comprehension of the human transcriptome

AU - Dalla, Emiliano

AU - Verardo, Roberto

AU - Lazarević, Dejan

AU - Marchionni, Luigi

AU - Reid, James F.

AU - Bahar, Nabil

AU - Klarić, Enio

AU - Marcuzzi, Giacomo

AU - Marzio, Riccardo

AU - Belgrano, Anna

AU - Licastro, Danilo

AU - Schneider, Claudio

N1 - Funding Information: This study was supported by NCI project UOICA 80200 and CNR project Functional Genomics – Bioinfomatics and Comparative Functional Genomics.

PY - 2003

Y1 - 2003

N2 - LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23 000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

AB - LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23 000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

KW - Full-length cDNA

KW - Functional analysis

KW - Human transcriptome

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DO - 10.1016/j.crvi.2003.09.029

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AN - SCOPUS:9144273280

SN - 1631-0691

VL - 326

SP - 967

EP - 970

JO - Comptes Rendus - Biologies

JF - Comptes Rendus - Biologies

IS - 10-11

ER -

LNCIB human full-length cDNAs collection: Towards a better comprehension of the human transcriptome

Abstract

Keywords

ASJC Scopus subject areas

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