Lipid/Peptide/Nucleotide Separation with MALDI-Ion Mobility-TOF MS

Amina S. Woods, Michael Ugarov, Tom Egan, John Koomen, Kent J. Gillig, Katrin Fuhrer, Marc Gonin, J. Albert Schultz

Research output: Contribution to journalArticlepeer-review

113 Scopus citations


Matrix-assisted laser desorption/ionization when combined with ion mobility-orthogonal time-of-flight mass spectrometry is a viable technique for fast separation and analysis of biomolecules in complex mixtures. Isobaric lipid, peptide, and oligonucleotide ions are preseparated before mass analysis by differences of up to 30% in mobility drift time. Ions of similar chemical type fall along well-defined "trend lines" (with deviations of ∼3%) when plotted in two-dimensional representations of ion mobility as a function of m/z. Discussion of fundamental and technical limitations of the technique point to its potential for being most useful when applied to systems such as bodily fluids and intact tissue, where an alternative chemical or chromatographic preseparation step prior to mass analysis is either impractical or undesirable.

Original languageEnglish (US)
Pages (from-to)2187-2195
Number of pages9
JournalAnalytical Chemistry
Issue number8
StatePublished - Apr 15 2004
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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