Linear viscoelastic moduli of concentrated DNA solutions

T. G. Mason; A. Dhople; D. Wirtz

doi:10.1021/ma970564w

Linear viscoelastic moduli of concentrated DNA solutions

T. G. Mason, A. Dhople, D. Wirtz

Whiting School of Engineering

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

We present mechanical measurements of the frequency-dependent linear viscoelastic storage and loss moduli, G′(ω) and G″(ω), for calf thymus DNA (13 kbp) in a saline buffer over a range of mitotically relevant mass concentrations from C = 1 to 10 mg/mL. For C exceeding a critical entanglement concentration, C_e, we observe a dominant plateau elasticity, G′_p, for large ω. As ω decreases, G′ falls until it is equal to G″ at the crossover frequency, ω_c, below which G″ dominates. These measurements are consistent with a model of entangled semiflexible polymer coils in a good solvent, which predicts G′_p ~∼C^2.3 and ω_c ∼ C^-2.4.

Original language	English (US)
Pages (from-to)	3600-3603
Number of pages	4
Journal	Macromolecules
Volume	31
Issue number	11
DOIs	https://doi.org/10.1021/ma970564w
State	Published - Jun 2 1998

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Inorganic Chemistry
Materials Chemistry

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abstract = "We present mechanical measurements of the frequency-dependent linear viscoelastic storage and loss moduli, G′(ω) and G″(ω), for calf thymus DNA (13 kbp) in a saline buffer over a range of mitotically relevant mass concentrations from C = 1 to 10 mg/mL. For C exceeding a critical entanglement concentration, Ce, we observe a dominant plateau elasticity, G′p, for large ω. As ω decreases, G′ falls until it is equal to G″ at the crossover frequency, ωc, below which G″ dominates. These measurements are consistent with a model of entangled semiflexible polymer coils in a good solvent, which predicts G′p ~∼C2.3 and ωc ∼ C-2.4.",

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AU - Mason, T. G.

AU - Dhople, A.

AU - Wirtz, D.

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N2 - We present mechanical measurements of the frequency-dependent linear viscoelastic storage and loss moduli, G′(ω) and G″(ω), for calf thymus DNA (13 kbp) in a saline buffer over a range of mitotically relevant mass concentrations from C = 1 to 10 mg/mL. For C exceeding a critical entanglement concentration, Ce, we observe a dominant plateau elasticity, G′p, for large ω. As ω decreases, G′ falls until it is equal to G″ at the crossover frequency, ωc, below which G″ dominates. These measurements are consistent with a model of entangled semiflexible polymer coils in a good solvent, which predicts G′p ~∼C2.3 and ωc ∼ C-2.4.

AB - We present mechanical measurements of the frequency-dependent linear viscoelastic storage and loss moduli, G′(ω) and G″(ω), for calf thymus DNA (13 kbp) in a saline buffer over a range of mitotically relevant mass concentrations from C = 1 to 10 mg/mL. For C exceeding a critical entanglement concentration, Ce, we observe a dominant plateau elasticity, G′p, for large ω. As ω decreases, G′ falls until it is equal to G″ at the crossover frequency, ωc, below which G″ dominates. These measurements are consistent with a model of entangled semiflexible polymer coils in a good solvent, which predicts G′p ~∼C2.3 and ωc ∼ C-2.4.

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Linear viscoelastic moduli of concentrated DNA solutions

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