TY - JOUR
T1 - LC-MS/MS Determination of Organophosphorus Pesticide Residues in Coconut Water
AU - Deme, Pragney
AU - Azmeera, Tirupathi
AU - Kanjilal, Sanjit
AU - Jonnalagadda, Padmaja
AU - Upadhyayula, Vijaya Saradhi
N1 - Funding Information:
Acknowledgments The authors thank Dr. J. S. Yadav, Director, IICT Dr. R. Srinivas, Head, NCMS, IICT, Dr. R.B.N. Prasad, Head, Lipid Science and Technology, IICT, and Dr. B. Sesikeran, Director, NIN, for their encouragement and support. The work has been carried out under the project CNP-0815. The frist author thanks the Council of Scientific and Industrial Research, (CSIR) New Delhi, India, for the award of Senior Research Fellowship (SRF).
PY - 2013/8
Y1 - 2013/8
N2 - A simple and specific analytical method based on solid phase extraction (SPE), and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) operated in multipule reaction monitoring (MRM) mode with dynamic time segment acquisition windows, was developed to determine organophosphorus pesticide residues (acephate, monocrotophos, dimethoate, malaoxon, dichlorvos, malathion, phenthoate, parathion-ethyl, chlorfenvinfos, quinalphos, diazinon, phosalone, profenofos, ethion, chlorpyrifos) in tender coconut water. The method was observed to be linear with lower limit of detections ranged from 0.1 to 1.5 ng/mL (measured at S/N ratio 5:1) and limit of qunatitation 0.5 to 2.0 ng/mL (measured at S/N ratio 10:1) for all studied pesticides. The intra- and inter-day assay accuracies calculated at four concentration levels in six replicates (n = 6) were in the range of 80 % to 115 % with relative standard deviations (RSD) below 15 %. The percentage recoveries of all the analysed pesticides were in the range of 86.8 % to 107.6 % and the calculated RSD values were below 12 %. The developed method was applied to determine pesticide residues in natural tender coconut water samples. The results showed the presence of malathion ranging from 24 to 45 ng/L concentrations in some natural tender coconut water samples collected from various local market places in and around Hyderabad, Andhra Pradesh, India.
AB - A simple and specific analytical method based on solid phase extraction (SPE), and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) operated in multipule reaction monitoring (MRM) mode with dynamic time segment acquisition windows, was developed to determine organophosphorus pesticide residues (acephate, monocrotophos, dimethoate, malaoxon, dichlorvos, malathion, phenthoate, parathion-ethyl, chlorfenvinfos, quinalphos, diazinon, phosalone, profenofos, ethion, chlorpyrifos) in tender coconut water. The method was observed to be linear with lower limit of detections ranged from 0.1 to 1.5 ng/mL (measured at S/N ratio 5:1) and limit of qunatitation 0.5 to 2.0 ng/mL (measured at S/N ratio 10:1) for all studied pesticides. The intra- and inter-day assay accuracies calculated at four concentration levels in six replicates (n = 6) were in the range of 80 % to 115 % with relative standard deviations (RSD) below 15 %. The percentage recoveries of all the analysed pesticides were in the range of 86.8 % to 107.6 % and the calculated RSD values were below 12 %. The developed method was applied to determine pesticide residues in natural tender coconut water samples. The results showed the presence of malathion ranging from 24 to 45 ng/L concentrations in some natural tender coconut water samples collected from various local market places in and around Hyderabad, Andhra Pradesh, India.
KW - Coconut water
KW - Liquid chromatography
KW - Mass spectrometry
KW - Organophosphorus pesticides
KW - Solid phase extraction
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U2 - 10.1007/s12161-012-9523-2
DO - 10.1007/s12161-012-9523-2
M3 - Article
AN - SCOPUS:84879792941
SN - 1936-9751
VL - 6
SP - 1162
EP - 1169
JO - Food Analytical Methods
JF - Food Analytical Methods
IS - 4
ER -