Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

Bi Chang Chen; Wesley R. Legant; Kai Wang; Lin Shao; Daniel E. Milkie; Michael W. Davidson; Chris Janetopoulos; Xufeng S. Wu; John A. Hammer; Zhe Liu; Brian P. English; Yuko Mimori-Kiyosue; Daniel P. Romero; Alex T. Ritter; Jennifer Lippincott-Schwartz; Lillian Fritz-Laylin; R. Dyche Mullins; Diana M. Mitchell; Joshua N. Bembenek; Anne Cecile Reymann; Ralph Böhme; Stephan W. Grill; Jennifer T. Wang; Geraldine Seydoux; U. Serdar Tulu; Daniel P. Kiehart; Eric Betzig

doi:10.1126/science.1257998

Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

Bi Chang Chen, Wesley R. Legant, Kai Wang, Lin Shao, Daniel E. Milkie, Michael W. Davidson, Chris Janetopoulos, Xufeng S. Wu, John A. Hammer, Zhe Liu, Brian P. English, Yuko Mimori-Kiyosue, Daniel P. Romero, Alex T. Ritter, Jennifer Lippincott-Schwartz, Lillian Fritz-Laylin, R. Dyche Mullins, Diana M. Mitchell, Joshua N. Bembenek, Anne Cecile ReymannRalph Böhme, Stephan W. Grill, Jennifer T. Wang, Geraldine Seydoux, U. Serdar Tulu, Daniel P. Kiehart, Eric Betzig

Howard Hughes Medical Institution

Research output: Contribution to journal › Article › peer-review

890 Scopus citations

Abstract

Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools.We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems.

Original language	English (US)
Article number	1257998
Journal	Science
Volume	346
Issue number	6208
DOIs	https://doi.org/10.1126/science.1257998
State	Published - Oct 24 2014

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Chen, B. C., Legant, W. R., Wang, K., Shao, L., Milkie, D. E., Davidson, M. W., Janetopoulos, C., Wu, X. S., Hammer, J. A., Liu, Z., English, B. P., Mimori-Kiyosue, Y., Romero, D. P., Ritter, A. T., Lippincott-Schwartz, J., Fritz-Laylin, L., Mullins, R. D., Mitchell, D. M., Bembenek, J. N., ... Betzig, E. (2014). Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution. Science, 346(6208), Article 1257998. https://doi.org/10.1126/science.1257998

Chen, BC, Legant, WR, Wang, K, Shao, L, Milkie, DE, Davidson, MW, Janetopoulos, C, Wu, XS, Hammer, JA, Liu, Z, English, BP, Mimori-Kiyosue, Y, Romero, DP, Ritter, AT, Lippincott-Schwartz, J, Fritz-Laylin, L, Mullins, RD, Mitchell, DM, Bembenek, JN, Reymann, AC, Böhme, R, Grill, SW, Wang, JT, Seydoux, G, Tulu, US, Kiehart, DP & Betzig, E 2014, 'Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution', Science, vol. 346, no. 6208, 1257998. https://doi.org/10.1126/science.1257998

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title = "Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution",

abstract = "Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools.We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems.",

author = "Chen, {Bi Chang} and Legant, {Wesley R.} and Kai Wang and Lin Shao and Milkie, {Daniel E.} and Davidson, {Michael W.} and Chris Janetopoulos and Wu, {Xufeng S.} and Hammer, {John A.} and Zhe Liu and English, {Brian P.} and Yuko Mimori-Kiyosue and Romero, {Daniel P.} and Ritter, {Alex T.} and Jennifer Lippincott-Schwartz and Lillian Fritz-Laylin and Mullins, {R. Dyche} and Mitchell, {Diana M.} and Bembenek, {Joshua N.} and Reymann, {Anne Cecile} and Ralph B{\"o}hme and Grill, {Stephan W.} and Wang, {Jennifer T.} and Geraldine Seydoux and Tulu, {U. Serdar} and Kiehart, {Daniel P.} and Eric Betzig",

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doi = "10.1126/science.1257998",

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T1 - Lattice light-sheet microscopy

T2 - Imaging molecules to embryos at high spatiotemporal resolution

AU - Chen, Bi Chang

AU - Legant, Wesley R.

AU - Wang, Kai

AU - Shao, Lin

AU - Milkie, Daniel E.

AU - Davidson, Michael W.

AU - Janetopoulos, Chris

AU - Wu, Xufeng S.

AU - Hammer, John A.

AU - Liu, Zhe

AU - English, Brian P.

AU - Mimori-Kiyosue, Yuko

AU - Romero, Daniel P.

AU - Ritter, Alex T.

AU - Lippincott-Schwartz, Jennifer

AU - Fritz-Laylin, Lillian

AU - Mullins, R. Dyche

AU - Mitchell, Diana M.

AU - Bembenek, Joshua N.

AU - Reymann, Anne Cecile

AU - Böhme, Ralph

AU - Grill, Stephan W.

AU - Wang, Jennifer T.

AU - Seydoux, Geraldine

AU - Tulu, U. Serdar

AU - Kiehart, Daniel P.

AU - Betzig, Eric

PY - 2014/10/24

Y1 - 2014/10/24

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AB - Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools.We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems.

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Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

Abstract

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