Abstract
The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 nonredundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.
Original language | English (US) |
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Pages (from-to) | 4274-4278 |
Number of pages | 5 |
Journal | Proteomics |
Volume | 11 |
Issue number | 21 |
DOIs | |
State | Published - Jan 1 2011 |
Keywords
- Glycoproteomics
- Hydrazide chemistry
- Insoluble and soluble protein fraction
- Ionic liquid
- N-glycoproteome
- Rat brain
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology