Large-scale N-glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions

Xiaoqiang Qiao; Dingyin Tao; Yanyan Qu; Liangliang Sun; Liang Gao; Xiaodan Zhang; Zhen Liang; Lihua Zhang; Yukui Zhang

doi:10.1002/pmic.201000715

Large-scale N-glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions

Xiaoqiang Qiao, Dingyin Tao, Yanyan Qu, Liangliang Sun, Liang Gao, Xiaodan Zhang, Zhen Liang, Lihua Zhang, Yukui Zhang

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 nonredundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.

Original language	English (US)
Pages (from-to)	4274-4278
Number of pages	5
Journal	Proteomics
Volume	11
Issue number	21
DOIs	https://doi.org/10.1002/pmic.201000715
State	Published - Nov 2011
Externally published	Yes

Keywords

Glycoproteomics
Hydrazide chemistry
Insoluble and soluble protein fraction
Ionic liquid
N-glycoproteome
Rat brain

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1002/pmic.201000715

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title = "Large-scale N-glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions",

abstract = "The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 nonredundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.",

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author = "Xiaoqiang Qiao and Dingyin Tao and Yanyan Qu and Liangliang Sun and Liang Gao and Xiaodan Zhang and Zhen Liang and Lihua Zhang and Yukui Zhang",

year = "2011",

month = nov,

doi = "10.1002/pmic.201000715",

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T2 - Simultaneous characterization of insoluble and soluble protein fractions

AU - Qiao, Xiaoqiang

AU - Tao, Dingyin

AU - Qu, Yanyan

AU - Sun, Liangliang

AU - Gao, Liang

AU - Zhang, Xiaodan

AU - Liang, Zhen

AU - Zhang, Lihua

AU - Zhang, Yukui

PY - 2011/11

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N2 - The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 nonredundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.

AB - The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 nonredundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.

KW - Glycoproteomics

KW - Hydrazide chemistry

KW - Insoluble and soluble protein fraction

KW - Ionic liquid

KW - N-glycoproteome

KW - Rat brain

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Large-scale N-glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions

Abstract

Keywords

ASJC Scopus subject areas

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