Large-scale N-glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions

Xiaoqiang Qiao; Dingyin Tao; Yanyan Qu; Liangliang Sun; Liang Gao; Xiaodan Zhang; Zhen Liang; Lihua Zhang; Yukui Zhang

doi:10.1002/pmic.201000715

Large-scale N-glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions

Xiaoqiang Qiao, Dingyin Tao, Yanyan Qu, Liangliang Sun, Liang Gao, Xiaodan Zhang, Zhen Liang, Lihua Zhang, Yukui Zhang

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 nonredundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.

Original language	English (US)
Pages (from-to)	4274-4278
Number of pages	5
Journal	Proteomics
Volume	11
Issue number	21
DOIs	https://doi.org/10.1002/pmic.201000715
State	Published - Jan 1 2011

Keywords

Glycoproteomics
Hydrazide chemistry
Insoluble and soluble protein fraction
Ionic liquid
N-glycoproteome
Rat brain

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1002/pmic.201000715

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title = "Large-scale N-glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions",

abstract = "The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 nonredundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.",

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T2 - Simultaneous characterization of insoluble and soluble protein fractions

AU - Qiao, Xiaoqiang

AU - Tao, Dingyin

AU - Qu, Yanyan

AU - Sun, Liangliang

AU - Gao, Liang

AU - Zhang, Xiaodan

AU - Liang, Zhen

AU - Zhang, Lihua

AU - Zhang, Yukui

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AB - The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 nonredundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.

KW - Glycoproteomics

KW - Hydrazide chemistry

KW - Insoluble and soluble protein fraction

KW - Ionic liquid

KW - N-glycoproteome

KW - Rat brain

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Large-scale N-glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions

Abstract

Keywords

ASJC Scopus subject areas

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