Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens

Nuntra Suwantarat; Justin B. Dalton; Richard Lee; Rachel Green; Warda Memon; Karen C. Carroll; Stefan Riedel; Sean X. Zhang

doi:10.1016/j.diagmicrobio.2015.01.012

Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens

Nuntra Suwantarat, Justin B. Dalton, Richard Lee, Rachel Green, Warda Memon, Karen C. Carroll, Stefan Riedel, Sean X. Zhang

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.

Original language	English (US)
Pages (from-to)	54-56
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2015.01.012
State	Published - May 1 2015

Keywords

Antigen
Cerebrospinal fluid
Cryptococcus
Lateral flow immunoassay
Serum

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/j.diagmicrobio.2015.01.012

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title = "Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens",

abstract = "We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.",

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AU - Suwantarat, Nuntra

AU - Dalton, Justin B.

AU - Lee, Richard

AU - Green, Rachel

AU - Memon, Warda

AU - Carroll, Karen C.

AU - Riedel, Stefan

AU - Zhang, Sean X.

PY - 2015/5/1

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N2 - We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.

AB - We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.

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KW - Cerebrospinal fluid

KW - Cryptococcus

KW - Lateral flow immunoassay

KW - Serum

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Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens

Abstract

Keywords

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