Abstract
This study examined the role of lactosylceramide (LacCer),a ubiquitous glycosphingolipid known to active NADPH oxidase, as a second messenger in the shear-induced O2- generatin. Superoxide generation was measured in sheared (20 dynes/cm2) human umbilical vein ECs by lucigenin chemiluminescence, and was found to peak at 30 min after the onset of shear (approx. 4-fold increase compared to static controls). EC pretreatment with D-PDMP, an inhibitor of the enzyme UDP-Gal:GlcCer, b(1>4)-galactosyltransferase (GAlT-2) that synthesizes LacCer from glucosylceramide (GlcCer), abolished the shear-induced increase in O2- production. EC pretreatment with the antioxidants N-acetylcysteine (NAC) or pyrolidine dithiocarbamate (PDTC) als suppressed the O2- production due to shear.
Original language | English (US) |
---|---|
Pages (from-to) | S-85 |
Journal | Annals of biomedical engineering |
Volume | 28 |
Issue number | SUPPL. 1 |
State | Published - 2000 |
Event | 2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA Duration: Oct 12 2000 → Oct 14 2000 |
ASJC Scopus subject areas
- Biomedical Engineering