Lactosylceramide mediates the shear-induced superoxide production in vascular endothelial cells

Li Hong Yeh, Adam M. Kinsey, Subroto Chatterjee, B. Rita Alevriadou

Research output: Contribution to journalConference articlepeer-review

Abstract

This study examined the role of lactosylceramide (LacCer),a ubiquitous glycosphingolipid known to active NADPH oxidase, as a second messenger in the shear-induced O2- generatin. Superoxide generation was measured in sheared (20 dynes/cm2) human umbilical vein ECs by lucigenin chemiluminescence, and was found to peak at 30 min after the onset of shear (approx. 4-fold increase compared to static controls). EC pretreatment with D-PDMP, an inhibitor of the enzyme UDP-Gal:GlcCer, b(1>4)-galactosyltransferase (GAlT-2) that synthesizes LacCer from glucosylceramide (GlcCer), abolished the shear-induced increase in O2- production. EC pretreatment with the antioxidants N-acetylcysteine (NAC) or pyrolidine dithiocarbamate (PDTC) als suppressed the O2- production due to shear.

Original languageEnglish (US)
Pages (from-to)S-85
JournalAnnals of biomedical engineering
Volume28
Issue numberSUPPL. 1
StatePublished - 2000
Event2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA
Duration: Oct 12 2000Oct 14 2000

ASJC Scopus subject areas

  • Biomedical Engineering

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