Lack of role of TGF-β1 in decreased lymphoproliferative response in HIV-1 infection

R. K. Chopra; N. B.K. Raj; J. P. Scally; W. R. Cappuccio; J. E. Nagel; A. J. Saah; J. B. Margolick

doi:10.1006/clin.1993.1152

Lack of role of TGF-β1 in decreased lymphoproliferative response in HIV-1 infection

R. K. Chopra, N. B.K. Raj, J. P. Scally, W. R. Cappuccio, J. E. Nagel, A. J. Saah, J. B. Margolick

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We investigated whether reduction of the phytohemagglutinin (PHA)-induced proliferative response of lymphocytes from HIV type-1 (HIV-1)-infected (HIV⁺) individuals could be explained by overproduction of transforming growth factor-β1 (TGF-β1), a strong inhibitor of T cell proliferation. PHA-stimulated PBMC from 40 HIV^- and 42 HIV⁺ homosexual men from the Baltimore Center of the Multicenter AIDS Cohort Study (MACS) were studied using Northern blot analysis of expression of TGF-β1 mRNA and determining the effects of anti-TGF-β1 neutralizing antibody on PHA-induced proliferative responses. Compared to the HIV^- donors, HIV⁺ donors did not show increased expression of TGF-β1 mRNA in unstimulated or PHA-stimulated PBMC. Furthermore, a neutralizing antibody to TGF-β1 did not reverse the decreased proliferative response of PBMC from HIV⁺ individuals to PHA or interleukin-2. These results indicate that TGF-β1 is not involved in T cell proliferation defects seen in HIV⁺ donors.

Original language	English (US)
Pages (from-to)	77-82
Number of pages	6
Journal	Clinical Immunology and Immunopathology
Volume	69
Issue number	1
DOIs	https://doi.org/10.1006/clin.1993.1152
State	Published - Oct 1993

ASJC Scopus subject areas

Immunology and Allergy
Pathology and Forensic Medicine
Immunology

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title = "Lack of role of TGF-β1 in decreased lymphoproliferative response in HIV-1 infection",

abstract = "We investigated whether reduction of the phytohemagglutinin (PHA)-induced proliferative response of lymphocytes from HIV type-1 (HIV-1)-infected (HIV+) individuals could be explained by overproduction of transforming growth factor-β1 (TGF-β1), a strong inhibitor of T cell proliferation. PHA-stimulated PBMC from 40 HIV- and 42 HIV+ homosexual men from the Baltimore Center of the Multicenter AIDS Cohort Study (MACS) were studied using Northern blot analysis of expression of TGF-β1 mRNA and determining the effects of anti-TGF-β1 neutralizing antibody on PHA-induced proliferative responses. Compared to the HIV- donors, HIV+ donors did not show increased expression of TGF-β1 mRNA in unstimulated or PHA-stimulated PBMC. Furthermore, a neutralizing antibody to TGF-β1 did not reverse the decreased proliferative response of PBMC from HIV+ individuals to PHA or interleukin-2. These results indicate that TGF-β1 is not involved in T cell proliferation defects seen in HIV+ donors.",

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AU - Scally, J. P.

AU - Cappuccio, W. R.

AU - Nagel, J. E.

AU - Saah, A. J.

AU - Margolick, J. B.

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N2 - We investigated whether reduction of the phytohemagglutinin (PHA)-induced proliferative response of lymphocytes from HIV type-1 (HIV-1)-infected (HIV+) individuals could be explained by overproduction of transforming growth factor-β1 (TGF-β1), a strong inhibitor of T cell proliferation. PHA-stimulated PBMC from 40 HIV- and 42 HIV+ homosexual men from the Baltimore Center of the Multicenter AIDS Cohort Study (MACS) were studied using Northern blot analysis of expression of TGF-β1 mRNA and determining the effects of anti-TGF-β1 neutralizing antibody on PHA-induced proliferative responses. Compared to the HIV- donors, HIV+ donors did not show increased expression of TGF-β1 mRNA in unstimulated or PHA-stimulated PBMC. Furthermore, a neutralizing antibody to TGF-β1 did not reverse the decreased proliferative response of PBMC from HIV+ individuals to PHA or interleukin-2. These results indicate that TGF-β1 is not involved in T cell proliferation defects seen in HIV+ donors.

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Lack of role of TGF-β1 in decreased lymphoproliferative response in HIV-1 infection

Abstract

ASJC Scopus subject areas

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