Abstract
We recently reported that a cloned murine cell line with "natural killer (NK)-like" cytolytic function and prominent cytoplasmic granules also expressed large numbers of plasma membrane receptors (Fcε{lunate}R) which bound mouse immunoglobulin E (IgE) with high affinity (S. J. Galli et al., 1982, Nature (London)298, 288). We have now performed IgE-binding studies with 31 additional cloned murine cell lines exhibiting "NK-like" lytic activity (defined as the ability to kill YAC-1 lymphoma cells) and three antigen-specific cytotoxic-T-cell clones. One of the NK-like clones expressed a small number of Fcε{lunate}R ( 3.0 × 104 cell) on one of the two occasions it was tested. None of the other clones, which were derived by several different approaches and which had a variety of surface glycoprotein phenotypes, expressed any detectable specific binding of mouse IgE. By contrast, mast cell clones consistently expressed large numbers of Fcε{lunate}R. The expression of large numbers of high-affinity Fcε{lunate}R would appear to represent a very uncommon characteristic of NK-like murine cell lines isolated under conditions similar to those described in this report.
Original language | English (US) |
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Pages (from-to) | 223-230 |
Number of pages | 8 |
Journal | Cellular Immunology |
Volume | 96 |
Issue number | 1 |
DOIs | |
State | Published - Nov 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology