Laboratory maintenance of rickettsia rickettsii

Nicole C. Ammerman, Magda Beier-Sexton, Abdu F. Azad

Research output: Contribution to journalReview articlepeer-review

42 Scopus citations


This unit includes protocols for the laboratory maintenance of the obligate intracellular bacterium Rickettsia rickettsii, including propagation in mammalian cell cultures, as isolation, counting, and storage procedures. Regulations for working with ettsii in biosafety level 3 containment are also discussed.

Original languageEnglish (US)
Pages (from-to)3A.5.1-3A.5.21
JournalCurrent Protocols in Microbiology
Issue numberSUPPL. 11
StatePublished - 2008
Externally publishedYes


  • Cell culture techniques
  • Rickettsia
  • Rocky Mountain spotted fever
  • Vero cells

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology


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