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Labeling of cells with silver and gold host-guest nanocomposites

  • Lajos P. Balogh
  • , Wojciech Lesniak
  • , Vladimir A. Sinani
  • , Anna U. Bielinska
  • , Kai Sun
  • , Bindu M. Nair
  • , Emily Waite
  • , Muhammed S.T. Kariapper
  • , Mohamed K. Khan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Nanocomposites combine the properties of nano-sized inorganic guest atoms/molecules with their nanoscopic size polymer host. Using poly(amidoamine) (PAMAM) dendrimers as templates we have synthesized water-soluble, biocompatible, fluorescent, and stable nanocomposites with regulated surface charge and controlled size for cell labeling studies. Silver {Ag 0-PAMAM} and gold {Au0PAMAM} dendrimer nanocomposites were used for in vitro cell labeling. The results indicate that surface charge plays a significant role in biocompatibility and cellular intemalization. Cytotoxicity (or lack thereof) of the host dendrimers and related nanocomposites was evaluated in normal and cancer cells using XTT assay. Cellular uptake of nanoparticles was examined by TEM and confocal microscopy. We demonstrate that nanocomposites can be used as cell biomarkers.

Original languageEnglish (US)
Title of host publication2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
Pages462-465
Number of pages4
StatePublished - 2006
Externally publishedYes
Event2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings - Boston, MA, United States
Duration: May 7 2006May 11 2006

Publication series

Name2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
Volume2

Other

Other2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
Country/TerritoryUnited States
CityBoston, MA
Period5/7/065/11/06

Keywords

  • Cell labeling
  • Poly(amidoamine) dendrimer
  • Silver and gold/dendrimer nanocomposite

ASJC Scopus subject areas

  • General Engineering

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