@inbook{7b6f97bd81194e13a6f7c3bd2adab166,
title = "Labeling a protein with fluorophores using NHS ester derivitization",
abstract = "N-hydroxysuccinimde (NHS) ester-mediated derivitization involves the reaction of this amine-reactive group with the primary amines of a protein or a biomolecule. Using NHS chemistry allows one to conjugate various fluorescent probes, biotin, and cross-linkers to primary amines. For example, we use NHS ester chemistry to fluorescently label the amino terminus of a protein with the dye, 5-(and-6)-carboxyfluorescein, succinimidyl ester (5(6)-FAM, SE).",
keywords = "Efficiency of labeling calculation, Fluorescent labeling of protein, Fluorophores, Lyophilized protein, NHS ester derivitization, UV spectrophotometer",
author = "Nanda, {Jagpreet S.} and Lorsch, {Jon R.}",
year = "2014",
month = jan,
day = "1",
doi = "10.1016/B978-0-12-420070-8.00008-8",
language = "English (US)",
isbn = "9780124200708",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "87--94",
booktitle = "Laboratory Methods in Enzymology",
}