Abstract
For more than a century, colorimetric and fluorescence staining have been the foundation of a broad range of key bioanalytical techniques. The dynamics of such staining processes, however, still remains largely unexplored. Weinvestigated the kinetics of fluorescence staining of two Gram-negative and two Gram-positive species with 3,3′-diethylthiacyanine (THIA) iodide.An increase in the THIAfluorescence quantum yield, induced by the bacterial dye uptake, was the principal reason for the observed emission enhancement. The fluorescence quantum yield of THIA depended on the media viscosity and not on themedia polarity, which suggested that themicroenvironment of the dyemolecules taken up by the cells was restrictive. The kinetics of fluorescence staining did not manifest a statistically significant dependence neither on the dye concentration, nor on the cell count. In the presence of surfactant additives, however, the fluorescence-enhancement kinetic patterns manifested species specificity with statistically significant discernibility.
Original language | English (US) |
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Pages (from-to) | 9756-9765 |
Number of pages | 10 |
Journal | Langmuir |
Volume | 26 |
Issue number | 12 |
DOIs | |
State | Published - Jun 15 2010 |
Externally published | Yes |
ASJC Scopus subject areas
- General Materials Science
- Condensed Matter Physics
- Surfaces and Interfaces
- Spectroscopy
- Electrochemistry