Kinetics characterization of ligand-receptor interactions using oblique-incidence reflectivity difference method

Shuang Liu; Guozhen Yang; Huibin Lu; Heng Zhu

doi:10.1007/978-1-4939-2617-6_5

Kinetics characterization of ligand-receptor interactions using oblique-incidence reflectivity difference method

Shuang Liu, Guozhen Yang, Huibin Lu, Heng Zhu

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The oblique-incidence reflectivity difference (OIRD) method is a novel optical biosensor which has recently been applied to label-free, real-time detection of molecular interactions in microarray format using a functionalized glass substrate. The capability of the OIRD to independently monitor association and dissociation kinetics enables real-time profiling of ligand-receptor interactions. The microarray format of the OIRD method makes it a practical platform for high-throughput, system-wide affinity profiling of the interactions of proteins, DNA, or whole cells with a diverse set of biomolecules.

Original language	English (US)
Title of host publication	Label-Free Biosensor Methods in Drug Discovery
Publisher	Springer New York
Pages	111-118
Number of pages	8
ISBN (Electronic)	9781493926176
ISBN (Print)	9781493926169
DOIs	https://doi.org/10.1007/978-1-4939-2617-6_5
State	Published - Apr 6 2015

Keywords

Binding kinetics
Biomolecular interactions
High-throughput
Label-free
Oblique- incidence reflectivity difference (OIRD)

ASJC Scopus subject areas

Medicine(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1007/978-1-4939-2617-6_5

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abstract = "The oblique-incidence reflectivity difference (OIRD) method is a novel optical biosensor which has recently been applied to label-free, real-time detection of molecular interactions in microarray format using a functionalized glass substrate. The capability of the OIRD to independently monitor association and dissociation kinetics enables real-time profiling of ligand-receptor interactions. The microarray format of the OIRD method makes it a practical platform for high-throughput, system-wide affinity profiling of the interactions of proteins, DNA, or whole cells with a diverse set of biomolecules.",

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AU - Liu, Shuang

AU - Yang, Guozhen

AU - Lu, Huibin

AU - Zhu, Heng

PY - 2015/4/6

Y1 - 2015/4/6

N2 - The oblique-incidence reflectivity difference (OIRD) method is a novel optical biosensor which has recently been applied to label-free, real-time detection of molecular interactions in microarray format using a functionalized glass substrate. The capability of the OIRD to independently monitor association and dissociation kinetics enables real-time profiling of ligand-receptor interactions. The microarray format of the OIRD method makes it a practical platform for high-throughput, system-wide affinity profiling of the interactions of proteins, DNA, or whole cells with a diverse set of biomolecules.

AB - The oblique-incidence reflectivity difference (OIRD) method is a novel optical biosensor which has recently been applied to label-free, real-time detection of molecular interactions in microarray format using a functionalized glass substrate. The capability of the OIRD to independently monitor association and dissociation kinetics enables real-time profiling of ligand-receptor interactions. The microarray format of the OIRD method makes it a practical platform for high-throughput, system-wide affinity profiling of the interactions of proteins, DNA, or whole cells with a diverse set of biomolecules.

KW - Binding kinetics

KW - Biomolecular interactions

KW - High-throughput

KW - Label-free

KW - Oblique- incidence reflectivity difference (OIRD)

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M3 - Chapter

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BT - Label-Free Biosensor Methods in Drug Discovery

PB - Springer New York

ER -

Kinetics characterization of ligand-receptor interactions using oblique-incidence reflectivity difference method

Abstract

Keywords

ASJC Scopus subject areas

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