Abstract
The oblique-incidence reflectivity difference (OIRD) method is a novel optical biosensor which has recently been applied to label-free, real-time detection of molecular interactions in microarray format using a functionalized glass substrate. The capability of the OIRD to independently monitor association and dissociation kinetics enables real-time profiling of ligand-receptor interactions. The microarray format of the OIRD method makes it a practical platform for high-throughput, system-wide affinity profiling of the interactions of proteins, DNA, or whole cells with a diverse set of biomolecules.
Original language | English (US) |
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Title of host publication | Label-Free Biosensor Methods in Drug Discovery |
Publisher | Springer New York |
Pages | 111-118 |
Number of pages | 8 |
ISBN (Electronic) | 9781493926176 |
ISBN (Print) | 9781493926169 |
DOIs | |
State | Published - Apr 6 2015 |
Keywords
- Binding kinetics
- Biomolecular interactions
- High-throughput
- Label-free
- Oblique- incidence reflectivity difference (OIRD)
ASJC Scopus subject areas
- Medicine(all)
- Pharmacology, Toxicology and Pharmaceutics(all)