TY - JOUR
T1 - Jaboticaba (Myrciaria jaboticaba) peel extracts induce reticulum stress and apoptosis in breast cancer cells
AU - da Silva-Maia, Juliana Kelly
AU - Nagalingam, Arumugam
AU - Cazarin, Cinthia Baú Betim
AU - Marostica Junior, Mário Roberto
AU - Sharma, Dipali
N1 - Funding Information:
This study was financed in part by the Coordination for the Improvement of Higher Education Personnel - Brazil (CAPES) - Finance Code 001; The National Council for Scientific and Technological Development – CNPq (grant number 142493/2013-9; 204088/2014-3; 403328/2016-0; 301496/2019-6) and The São Paulo Research Foundation - FAPESP (grant number 2015/50333-1; 2018/11069-5; 2015/13320-9, 2019/13465-8).
Publisher Copyright:
© 2023 The Author(s)
PY - 2023/7/30
Y1 - 2023/7/30
N2 - Jaboticaba peel (Myrciaria jaboticaba) is a source of bioactive compounds. We investigated the anticancer activity of ethyl acetate extract (JE1) and hydroethanolic extract (JE2) of Jaboticaba peel against breast cancer. Both JE1 and JE2 inhibited clonogenic potential of MDA-MB-231 cells while JE1 was particularly effective in MCF7 cells. Anchorage-independent growth and cell viability was also inhibited by JE1 and JE2. In addition to growth inhibition, JE1 and JE2 could also inhibit migration and invasion of cells. Interestingly, JE1 and JE2 show selective inhibition towards certain breast cancer cells and biological processes. Mechanistic evaluations showed that JE1 induced PARP cleavage, BAX and BIP indicating apoptotic induction. An elevation of phosphorylated ERK was observed in MCF7 cells in response to JE1 and JE2 along with increased IRE-α and CHOP expression indicating increased endoplasmic stress. Therefore, Jaboticaba peel extracts could be potentially considered for further development for breast cancer inhibition.
AB - Jaboticaba peel (Myrciaria jaboticaba) is a source of bioactive compounds. We investigated the anticancer activity of ethyl acetate extract (JE1) and hydroethanolic extract (JE2) of Jaboticaba peel against breast cancer. Both JE1 and JE2 inhibited clonogenic potential of MDA-MB-231 cells while JE1 was particularly effective in MCF7 cells. Anchorage-independent growth and cell viability was also inhibited by JE1 and JE2. In addition to growth inhibition, JE1 and JE2 could also inhibit migration and invasion of cells. Interestingly, JE1 and JE2 show selective inhibition towards certain breast cancer cells and biological processes. Mechanistic evaluations showed that JE1 induced PARP cleavage, BAX and BIP indicating apoptotic induction. An elevation of phosphorylated ERK was observed in MCF7 cells in response to JE1 and JE2 along with increased IRE-α and CHOP expression indicating increased endoplasmic stress. Therefore, Jaboticaba peel extracts could be potentially considered for further development for breast cancer inhibition.
KW - Antitumor
KW - Bioactive compounds
KW - Breast cancer
KW - Myrciaria jaboticaba
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U2 - 10.1016/j.fochms.2023.100167
DO - 10.1016/j.fochms.2023.100167
M3 - Article
C2 - 36875800
AN - SCOPUS:85148861861
SN - 2666-5662
VL - 6
JO - Food Chemistry: Molecular Sciences
JF - Food Chemistry: Molecular Sciences
M1 - 100167
ER -