ITIH3 Is a potential biomarker for early detection of gastric cancer

Poh Kuan Chong; Huiyin Lee; Jianbiao Zhou; Shaw Cheng Liu; Marie Chiew Shia Loh; Ting Ting Wang; Siew Pang Chan; Duane T. Smoot; Hassan Ashktorab; Jimmy Bok Yan So; Khong Hee Lim; Khay Guan Yeoh; Yoon Pin Lim

doi:10.1021/pr100192h

ITIH3 Is a potential biomarker for early detection of gastric cancer

Poh Kuan Chong, Huiyin Lee, Jianbiao Zhou, Shaw Cheng Liu, Marie Chiew Shia Loh, Ting Ting Wang, Siew Pang Chan, Duane T. Smoot, Hassan Ashktorab, Jimmy Bok Yan So, Khong Hee Lim, Khay Guan Yeoh, Yoon Pin Lim

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Gastric cancer has one of the highest morbidities and mortalities worldwide. Early detection is key measure to improve the outcome of gastric cancer patients. In our efforts to identify potential markers for gastric cancer detection, we coupled xenotransplantation mouse model with a plasma proteomic approach. MKN45 gastric cancer cells were subcutaneously injected into nude mice and plasma samples from mice bearing different sizes of tumors were collected and subjected to iTRAQ and mass spectrometry analysis. ITIH3 protein was found to be more highly expressed in plasma of tumor bearing mice compared to control. Subsequent screening of ITIH3 expression in 167 clinical plasma samples, including 83 cancer-free subjects and 84 gastric cancer patients, revealed higher ITIH3 level in the plasma of gastric cancer patients. A receiver operating characteristics (ROC) curve estimated a maximal sensitivity of 96% at 66% specificity for ITIH3 in gastric cancer detection. In addition, plasma from early stage gastric cancer patient has significantly (p < 0.001) higher level of ITIH3 compared to that from noncancer subject. Our data suggest that ITIH3 may be a useful biomarker for early detection of gastric cancer.

Original language	English (US)
Pages (from-to)	3671-3679
Number of pages	9
Journal	Journal of proteome research
Volume	9
Issue number	7
DOIs	https://doi.org/10.1021/pr100192h
State	Published - Jul 2 2010
Externally published	Yes

Keywords

ITIH3
gastric cancer
mouse xenograft
plasma
proteomics

ASJC Scopus subject areas

Biochemistry
General Chemistry

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10.1021/pr100192h

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title = "ITIH3 Is a potential biomarker for early detection of gastric cancer",

abstract = "Gastric cancer has one of the highest morbidities and mortalities worldwide. Early detection is key measure to improve the outcome of gastric cancer patients. In our efforts to identify potential markers for gastric cancer detection, we coupled xenotransplantation mouse model with a plasma proteomic approach. MKN45 gastric cancer cells were subcutaneously injected into nude mice and plasma samples from mice bearing different sizes of tumors were collected and subjected to iTRAQ and mass spectrometry analysis. ITIH3 protein was found to be more highly expressed in plasma of tumor bearing mice compared to control. Subsequent screening of ITIH3 expression in 167 clinical plasma samples, including 83 cancer-free subjects and 84 gastric cancer patients, revealed higher ITIH3 level in the plasma of gastric cancer patients. A receiver operating characteristics (ROC) curve estimated a maximal sensitivity of 96% at 66% specificity for ITIH3 in gastric cancer detection. In addition, plasma from early stage gastric cancer patient has significantly (p < 0.001) higher level of ITIH3 compared to that from noncancer subject. Our data suggest that ITIH3 may be a useful biomarker for early detection of gastric cancer.",

keywords = "ITIH3, gastric cancer, mouse xenograft, plasma, proteomics",

author = "Chong, {Poh Kuan} and Huiyin Lee and Jianbiao Zhou and Liu, {Shaw Cheng} and Loh, {Marie Chiew Shia} and Wang, {Ting Ting} and Chan, {Siew Pang} and Smoot, {Duane T.} and Hassan Ashktorab and So, {Jimmy Bok Yan} and Lim, {Khong Hee} and Yeoh, {Khay Guan} and Lim, {Yoon Pin}",

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doi = "10.1021/pr100192h",

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AU - Chong, Poh Kuan

AU - Lee, Huiyin

AU - Zhou, Jianbiao

AU - Liu, Shaw Cheng

AU - Loh, Marie Chiew Shia

AU - Wang, Ting Ting

AU - Chan, Siew Pang

AU - Smoot, Duane T.

AU - Ashktorab, Hassan

AU - So, Jimmy Bok Yan

AU - Lim, Khong Hee

AU - Yeoh, Khay Guan

AU - Lim, Yoon Pin

PY - 2010/7/2

Y1 - 2010/7/2

N2 - Gastric cancer has one of the highest morbidities and mortalities worldwide. Early detection is key measure to improve the outcome of gastric cancer patients. In our efforts to identify potential markers for gastric cancer detection, we coupled xenotransplantation mouse model with a plasma proteomic approach. MKN45 gastric cancer cells were subcutaneously injected into nude mice and plasma samples from mice bearing different sizes of tumors were collected and subjected to iTRAQ and mass spectrometry analysis. ITIH3 protein was found to be more highly expressed in plasma of tumor bearing mice compared to control. Subsequent screening of ITIH3 expression in 167 clinical plasma samples, including 83 cancer-free subjects and 84 gastric cancer patients, revealed higher ITIH3 level in the plasma of gastric cancer patients. A receiver operating characteristics (ROC) curve estimated a maximal sensitivity of 96% at 66% specificity for ITIH3 in gastric cancer detection. In addition, plasma from early stage gastric cancer patient has significantly (p < 0.001) higher level of ITIH3 compared to that from noncancer subject. Our data suggest that ITIH3 may be a useful biomarker for early detection of gastric cancer.

AB - Gastric cancer has one of the highest morbidities and mortalities worldwide. Early detection is key measure to improve the outcome of gastric cancer patients. In our efforts to identify potential markers for gastric cancer detection, we coupled xenotransplantation mouse model with a plasma proteomic approach. MKN45 gastric cancer cells were subcutaneously injected into nude mice and plasma samples from mice bearing different sizes of tumors were collected and subjected to iTRAQ and mass spectrometry analysis. ITIH3 protein was found to be more highly expressed in plasma of tumor bearing mice compared to control. Subsequent screening of ITIH3 expression in 167 clinical plasma samples, including 83 cancer-free subjects and 84 gastric cancer patients, revealed higher ITIH3 level in the plasma of gastric cancer patients. A receiver operating characteristics (ROC) curve estimated a maximal sensitivity of 96% at 66% specificity for ITIH3 in gastric cancer detection. In addition, plasma from early stage gastric cancer patient has significantly (p < 0.001) higher level of ITIH3 compared to that from noncancer subject. Our data suggest that ITIH3 may be a useful biomarker for early detection of gastric cancer.

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KW - mouse xenograft

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KW - proteomics

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ITIH3 Is a potential biomarker for early detection of gastric cancer

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