ITC: More Than Just Binding Affinities

Ernesto Freire

doi:10.1002/9780470979129.ch20

ITC: More Than Just Binding Affinities

Krieger School of Arts and Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Original language	English (US)
Title of host publication	Label-Free Technologies for Drug Discovery
Publisher	John Wiley and Sons
Pages	303-312
Number of pages	10
ISBN (Print)	9780470746837
DOIs	https://doi.org/10.1002/9780470979129.ch20
State	Published - Feb 24 2011

Keywords

Enthalpy and entropy, forces of binding - two forces, determining ligand to target binding
Experimental observable in ITC - heat of reaction, binding enthalpy and entropy for reaction
Heat associated with each injection - proportional to amount of complex formed
ITC - more than just binding affinities
ITC, measuring binding enthalpy - and binding entropy, tool for optimization of drug candidates
Isothermal titration calorimetry (ITC) - gold standard, for measuring binding affinities
Standard ITC titration experiments - binding reaction, injection of second reactant into reaction cell
Thermodynamic signature - of candidate for optimization, measured by ITC
Thermodynamic signatures - for rosuvastatin binding to HMG-CoA reductase

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

Access to Document

10.1002/9780470979129.ch20

Cite this

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title = "ITC: More Than Just Binding Affinities",

keywords = "Enthalpy and entropy, forces of binding - two forces, determining ligand to target binding, Experimental observable in ITC - heat of reaction, binding enthalpy and entropy for reaction, Heat associated with each injection - proportional to amount of complex formed, ITC - more than just binding affinities, ITC, measuring binding enthalpy - and binding entropy, tool for optimization of drug candidates, Isothermal titration calorimetry (ITC) - gold standard, for measuring binding affinities, Standard ITC titration experiments - binding reaction, injection of second reactant into reaction cell, Thermodynamic signature - of candidate for optimization, measured by ITC, Thermodynamic signatures - for rosuvastatin binding to HMG-CoA reductase",

author = "Ernesto Freire",

year = "2011",

month = feb,

day = "24",

doi = "10.1002/9780470979129.ch20",

language = "English (US)",

isbn = "9780470746837",

pages = "303--312",

booktitle = "Label-Free Technologies for Drug Discovery",

publisher = "John Wiley and Sons",

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TY - CHAP

T1 - ITC

T2 - More Than Just Binding Affinities

AU - Freire, Ernesto

PY - 2011/2/24

Y1 - 2011/2/24

KW - Enthalpy and entropy, forces of binding - two forces, determining ligand to target binding

KW - Experimental observable in ITC - heat of reaction, binding enthalpy and entropy for reaction

KW - Heat associated with each injection - proportional to amount of complex formed

KW - ITC - more than just binding affinities

KW - ITC, measuring binding enthalpy - and binding entropy, tool for optimization of drug candidates

KW - Isothermal titration calorimetry (ITC) - gold standard, for measuring binding affinities

KW - Standard ITC titration experiments - binding reaction, injection of second reactant into reaction cell

KW - Thermodynamic signature - of candidate for optimization, measured by ITC

KW - Thermodynamic signatures - for rosuvastatin binding to HMG-CoA reductase

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M3 - Chapter

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EP - 312

BT - Label-Free Technologies for Drug Discovery

PB - John Wiley and Sons

ER -

ITC: More Than Just Binding Affinities

Keywords

ASJC Scopus subject areas

Access to Document

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