ITC: More Than Just Binding Affinities

Research output: Chapter in Book/Report/Conference proceedingChapter

Original languageEnglish (US)
Title of host publicationLabel-Free Technologies for Drug Discovery
PublisherJohn Wiley and Sons
Number of pages10
ISBN (Print)9780470746837
StatePublished - Feb 24 2011


  • Enthalpy and entropy, forces of binding - two forces, determining ligand to target binding
  • Experimental observable in ITC - heat of reaction, binding enthalpy and entropy for reaction
  • Heat associated with each injection - proportional to amount of complex formed
  • ITC - more than just binding affinities
  • ITC, measuring binding enthalpy - and binding entropy, tool for optimization of drug candidates
  • Isothermal titration calorimetry (ITC) - gold standard, for measuring binding affinities
  • Standard ITC titration experiments - binding reaction, injection of second reactant into reaction cell
  • Thermodynamic signature - of candidate for optimization, measured by ITC
  • Thermodynamic signatures - for rosuvastatin binding to HMG-CoA reductase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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