ISWI, a member of the SWl2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor

Toshio Tsukiyama; Carla Daniel; John Tamkun; Carl Wu

doi:10.1016/0092-8674(95)90217-1

ISWI, a member of the SWl2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor

Toshio Tsukiyama, Carla Daniel, John Tamkun, Carl Wu

Research output: Contribution to journal › Article › peer-review

Abstract

The generation of an accessible heat shock promoter in chromatin in vitro requires the concerted action of the GAGA transcription factor and NURF, an ATP-dependent nucleosome remodeling factor. NURF is composed of four subunits and is biochemically distinct from the SWI2/SNF2 multiprotein complex, a transcriptional activator that also appears to alter nucleosome structure. We have obtained protein microsequence and immunological evidence identifying the 140 kDa subunit of NURF as ISWI, previously of unknown function but highly related to SWI2/SNF2 only in the ATPase domain. The ISWI protein is localized to the cell nucleus and is expressed throughout Drosophila development at levels as high as 100,000 molecules/cell. The convergence of biochemical and genetic studies on ISWI and SWI2/SNF2 underscores these ATPases and their close relatives as key components of independent systems for chromatin remodeling.

Original language	English (US)
Pages (from-to)	1021-1026
Number of pages	6
Journal	Cell
Volume	83
Issue number	6
DOIs	https://doi.org/10.1016/0092-8674(95)90217-1
State	Published - Dec 15 1995
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1016/0092-8674(95)90217-1

Cite this

@article{d120a8be62a34c9fac1274010345b32c,

title = "ISWI, a member of the SWl2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor",

abstract = "The generation of an accessible heat shock promoter in chromatin in vitro requires the concerted action of the GAGA transcription factor and NURF, an ATP-dependent nucleosome remodeling factor. NURF is composed of four subunits and is biochemically distinct from the SWI2/SNF2 multiprotein complex, a transcriptional activator that also appears to alter nucleosome structure. We have obtained protein microsequence and immunological evidence identifying the 140 kDa subunit of NURF as ISWI, previously of unknown function but highly related to SWI2/SNF2 only in the ATPase domain. The ISWI protein is localized to the cell nucleus and is expressed throughout Drosophila development at levels as high as 100,000 molecules/cell. The convergence of biochemical and genetic studies on ISWI and SWI2/SNF2 underscores these ATPases and their close relatives as key components of independent systems for chromatin remodeling.",

author = "Toshio Tsukiyama and Carla Daniel and John Tamkun and Carl Wu",

note = "Funding Information: Correspondence should be addressed to C. W. or J. T. We thank Claude Klee for performing microsequence analysis on NURF peptides, Ryuji Kobayashi for protocols on in-gel proteolytic cleavage and HPLC of peptides, and Mike Fritsch for instruction on microbore HPLC. This work was supported by the Intramural Research Program of the National Cancer Institute (C. W.) and by Basic Research Grant FY94-0536 from the March of Dimes Birth Defects Foundation and National Institutes of Health grant ROl GM49883 to J. T.",

year = "1995",

month = dec,

day = "15",

doi = "10.1016/0092-8674(95)90217-1",

language = "English (US)",

volume = "83",

pages = "1021--1026",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "6",

}

TY - JOUR

T1 - ISWI, a member of the SWl2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor

AU - Tsukiyama, Toshio

AU - Daniel, Carla

AU - Tamkun, John

AU - Wu, Carl

N1 - Funding Information: Correspondence should be addressed to C. W. or J. T. We thank Claude Klee for performing microsequence analysis on NURF peptides, Ryuji Kobayashi for protocols on in-gel proteolytic cleavage and HPLC of peptides, and Mike Fritsch for instruction on microbore HPLC. This work was supported by the Intramural Research Program of the National Cancer Institute (C. W.) and by Basic Research Grant FY94-0536 from the March of Dimes Birth Defects Foundation and National Institutes of Health grant ROl GM49883 to J. T.

PY - 1995/12/15

Y1 - 1995/12/15

N2 - The generation of an accessible heat shock promoter in chromatin in vitro requires the concerted action of the GAGA transcription factor and NURF, an ATP-dependent nucleosome remodeling factor. NURF is composed of four subunits and is biochemically distinct from the SWI2/SNF2 multiprotein complex, a transcriptional activator that also appears to alter nucleosome structure. We have obtained protein microsequence and immunological evidence identifying the 140 kDa subunit of NURF as ISWI, previously of unknown function but highly related to SWI2/SNF2 only in the ATPase domain. The ISWI protein is localized to the cell nucleus and is expressed throughout Drosophila development at levels as high as 100,000 molecules/cell. The convergence of biochemical and genetic studies on ISWI and SWI2/SNF2 underscores these ATPases and their close relatives as key components of independent systems for chromatin remodeling.

AB - The generation of an accessible heat shock promoter in chromatin in vitro requires the concerted action of the GAGA transcription factor and NURF, an ATP-dependent nucleosome remodeling factor. NURF is composed of four subunits and is biochemically distinct from the SWI2/SNF2 multiprotein complex, a transcriptional activator that also appears to alter nucleosome structure. We have obtained protein microsequence and immunological evidence identifying the 140 kDa subunit of NURF as ISWI, previously of unknown function but highly related to SWI2/SNF2 only in the ATPase domain. The ISWI protein is localized to the cell nucleus and is expressed throughout Drosophila development at levels as high as 100,000 molecules/cell. The convergence of biochemical and genetic studies on ISWI and SWI2/SNF2 underscores these ATPases and their close relatives as key components of independent systems for chromatin remodeling.

UR - http://www.scopus.com/inward/record.url?scp=0029618369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029618369&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(95)90217-1

DO - 10.1016/0092-8674(95)90217-1

M3 - Article

C2 - 8521502

AN - SCOPUS:0029618369

SN - 0092-8674

VL - 83

SP - 1021

EP - 1026

JO - Cell

JF - Cell

IS - 6

ER -

ISWI, a member of the SWl2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this