@inbook{cb2d2741a56e4858837cc23564a4af5c,
title = "Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry",
abstract = "Perivascular cells represent an in vivo counterpart of mesenchymal stromal/stem cells that populate the outer layer of blood vessels. Pericytes in capillaries and microvessels and adventitial cells of large arteries and veins give rise to stem/progenitor cells when isolated and cultured in vitro. These cells have been considered candidate cell types for cell therapy. Adipose tissue, being highly vascularized, dispensable, and easily accessed, is a viable option to obtain perivascular cells for use in research and in clinical trials. Here, we describe our established protocol to extract perivascular cells from human fat through fluorescence-activated cell sorting, which allows for the isolation of defined populations of progenitor cells with high reproducibility.",
keywords = "Adipose tissue, Adventitial cell, Cell therapy, Flow cytometry, Mesenchymal stromal/stem cell, Pericyte, Perivascular stem cell",
author = "Neelima Thottappillil and Gomez-Salazar, {Mario A.} and Mary Archer and Bruno P{\'e}ault and James, {Aaron W.}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3762-3_3",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "25--33",
booktitle = "Methods in Molecular Biology",
}