Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry

Neelima Thottappillil, Mario A. Gomez-Salazar, Mary Archer, Bruno Péault, Aaron W. James

Research output: Chapter in Book/Report/Conference proceedingChapter


Perivascular cells represent an in vivo counterpart of mesenchymal stromal/stem cells that populate the outer layer of blood vessels. Pericytes in capillaries and microvessels and adventitial cells of large arteries and veins give rise to stem/progenitor cells when isolated and cultured in vitro. These cells have been considered candidate cell types for cell therapy. Adipose tissue, being highly vascularized, dispensable, and easily accessed, is a viable option to obtain perivascular cells for use in research and in clinical trials. Here, we describe our established protocol to extract perivascular cells from human fat through fluorescence-activated cell sorting, which allows for the isolation of defined populations of progenitor cells with high reproducibility.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Adipose tissue
  • Adventitial cell
  • Cell therapy
  • Flow cytometry
  • Mesenchymal stromal/stem cell
  • Pericyte
  • Perivascular stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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