Isolation of Kinetoplast DNA

Theresa A. Shapiro, Viiu A. Klein, Paul T. Englund

Research output: Chapter in Book/Report/Conference proceedingChapter


The kinetoplast DNA (kDNA) network of the protozoan parasite Crithidia fasciculata is a naturally occurring gigantic catenane containing several thousand DNA minicircles. Because of its unusual structure, kDNA is an excellent substrate to use in decatenation assays of topoisomerase activity. Beyond this application, kDNA has also proven a fascinating experimental subject in its own right. Studies of the unique structure, mechanism of replication, and genetic function of kDNA have led to numerous interesting discoveries (seerefs.1-5 for reviews).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages7
StatePublished - 1999

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Beckman SW28 Rotor
  • CsCl Gradient
  • Gentian Violet
  • Kinetoplastid Parasite
  • Sucrose Cushion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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