@article{fff2735b85c940abababb570aa24af68,
title = " Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreER T2 transgenic piglet ",
abstract = " Background: The porcine brain is gyrencephalic with similar gray and white matter composition and size more comparable to the human rather than the rodent brain; however, there is lack of information about neural progenitor cells derived from this model. Results: Here, we isolated GFAP-positive porcine neural stem cells (NSCs) from the brain explant of a transgenic piglet, with expression of CreER T2 under the control of the GFAP promoter (pGFAP-CreER T2 ). The isolated pGFAP-CreER T2 NSCs showed self-renewal and expression of representative NSC markers such as Nestin and Sox2. Pharmacological inhibition studies revealed that Notch1 signaling is necessary to maintain NSC identity, whereas serum treatment induced cell differentiation into reactive astrocytes and neurons. Conclusions: Collectively, these results indicate that GFAP promoter-driven porcine CreER T2 NSCs would be a useful tool to study neurogenesis of the porcine adult central nervous system and furthers our understanding of its potential clinical application in the future. ",
keywords = "Neural stem cells, Notch signaling, Pig, Reactive astrocytes",
author = "Eunhye Kim and Hwang, {Seon Ung} and Yoon, {Junchul David} and Hyunggee Kim and Gabsang Lee and Hyun, {Sang Hwan}",
note = "Funding Information: This work was supported, in part, by a grant from the “National Research Foundation of Korea Grant funded by the Korean Government (NRF-2016R1D1A1B03933191, NRF-2017R1A2B4002546)”, “Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Advanced Production Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant number: 115103-02)”, “The Global Research and Development Center (GRDC) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2017K1A4A3014959)” and “Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration in 2017 (Grants No. 2017020681010101)”, Republic of Korea. The funder had no role in the design of the study and collection, analysis, and interpretation of data and in writing this manuscript. Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = nov,
day = "7",
doi = "10.1186/s12917-018-1660-4",
language = "English (US)",
volume = "14",
journal = "BMC veterinary research",
issn = "1746-6148",
publisher = "BioMed Central",
number = "1",
}