The predominant allergen from ragweed pollen has been characterized as a protein of 37,800 molecular weight by physicochemical and immunochemical procedures. The active protein constitutes about 6% of the proteins in the pollen extract and it has been designated as antigen E. Antigen E can be isolated in four chemical forms. These forms have identical molecular weights and amino acid compositions but they differ in their charges. On removal of antigen E from pollen extract by precipitation with specific rabbit antiserum, a 40-fold decrease of the allergenic activity was observed by direct skin tests of the supernatant on eight sensitive patients. The finding suggests that antigen E represents at least 90 % of the allergenic activity in ragweed pollen.
ASJC Scopus subject areas