TY - JOUR
T1 - Isolation and characterization of a mouse gastrin cDNA clone that expresses mast-cell growth-factor activity in monkey cells
AU - Yokota, T.
AU - Lee, F.
AU - Rennick, D.
AU - Hall, C.
AU - Arai, N.
AU - Mosmann, T.
AU - Nabel, G.
AU - Cantor, H.
AU - Arai, K.
PY - 1984
Y1 - 1984
N2 - A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF activity. The MCGF amino acid sequence between amino acids 33 and 41, deduced from the nucleotide sequence of its cDNA, is identical to the NH2-terminal sequence of the mouse lymphokine, interleukin 3, suggesting that MCGF is related to interleukin 3.
AB - A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF activity. The MCGF amino acid sequence between amino acids 33 and 41, deduced from the nucleotide sequence of its cDNA, is identical to the NH2-terminal sequence of the mouse lymphokine, interleukin 3, suggesting that MCGF is related to interleukin 3.
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U2 - 10.1073/pnas.81.4.1070
DO - 10.1073/pnas.81.4.1070
M3 - Article
C2 - 6322187
AN - SCOPUS:0642345766
SN - 0027-8424
VL - 81
SP - 1070
EP - 1074
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 4 I
ER -