Iron deficiency alters dopamine uptake and response to L-DOPA injection in Sprague-Dawley rats

Laura E. Bianco, Jason Wiesinger, Christopher J. Earley, Byron C. Jones, John L. Beard

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Iron deficiency (ID) disrupts brain dopamine (DA) and norepinephrine (NE) metabolism including functioning of monoamine transporters and receptors. We employed caudate microdialysis and no net flux (NNF) in post-weaning rats to determine if ID decreased the extraction fraction (Ed). Five micromolar quinpirole, a dopamine D2 receptor agonist, resulted in 80% decrease in extracellular DA and 45% higher Ed in control animals. The D2 agonist had no effect on Ed in ID animals despite a reduction in basal DA. DAT mRNA levels were reduced by 58% with ID, while DAT protein in ventral midbrain and caudate and membrane associated DAT were also reduced by ID. Carbidopa/l-DOPA was administered to determine if elevated extracellular DA in ID was due to increased release. The DA response to l-DOPA in ID rats was 50% smaller and delayed, whereas the NE response was threefold higher. The caudate concentration of NE was also elevated in ID. Elevated dopamine-β-hydroxylase activity in ID provides a tentative explanation for the increased NE response to l-DOPA. These experiments provide new evidence that ID results in altered synthesis and functioning of DAT and perhaps suggests some compensatory changes in NE metabolism.

Original languageEnglish (US)
Pages (from-to)205-215
Number of pages11
JournalJournal of Neurochemistry
Issue number1
StatePublished - Jul 2008


  • Brain iron
  • Dopamine
  • Dopamine transporter
  • Iron deficiency
  • L-DOPA
  • Microdialysis
  • Norepinephrine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Iron deficiency alters dopamine uptake and response to L-DOPA injection in Sprague-Dawley rats'. Together they form a unique fingerprint.

Cite this