IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry

Amina S. Woods, Micheal Ugarov, Shelley N. Jackson, Thomas Egan, Hay Yan J Wang, Kermit K. Murray, J. Albert Schultz

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Most MALDI instrumentation uses UV lasers. We have designed a MALDI-IM-oTOF-MS which employs both a Nd:YAG laser pumped optical parametric oscillator OPOTEK, λ = 2.8-3.2 μm at 20 Hz) to perform IR-LDI or IR-MALDI and a Nd:YLF laser (Crystalaser, λ = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined "trend lines". Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR-LDI produced similar results as IR-MALDI for the direct tissue analysis of phospholipids from rat brain.

Original languageEnglish (US)
Pages (from-to)1484-1487
Number of pages4
JournalJournal of Proteome Research
Issue number6
StatePublished - Jun 2006
Externally publishedYes


  • Adducts reduction
  • Ion-mobility MALDI
  • IR lasers
  • UV lasers

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry


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