IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry

Amina S. Woods; Micheal Ugarov; Shelley N. Jackson; Thomas Egan; Hay Yan J Wang; Kermit K. Murray; J. Albert Schultz

doi:10.1021/pr060055l

IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry

Amina S. Woods, Micheal Ugarov, Shelley N. Jackson, Thomas Egan, Hay Yan J Wang, Kermit K. Murray, J. Albert Schultz

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Most MALDI instrumentation uses UV lasers. We have designed a MALDI-IM-oTOF-MS which employs both a Nd:YAG laser pumped optical parametric oscillator OPOTEK, λ = 2.8-3.2 μm at 20 Hz) to perform IR-LDI or IR-MALDI and a Nd:YLF laser (Crystalaser, λ = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined "trend lines". Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR-LDI produced similar results as IR-MALDI for the direct tissue analysis of phospholipids from rat brain.

Original language	English (US)
Pages (from-to)	1484-1487
Number of pages	4
Journal	Journal of Proteome Research
Volume	5
Issue number	6
DOIs	https://doi.org/10.1021/pr060055l
State	Published - Jun 2006
Externally published	Yes

Keywords

Adducts reduction
Ion-mobility MALDI
IR lasers
UV lasers

ASJC Scopus subject areas

Genetics
Biotechnology
Biochemistry

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10.1021/pr060055l

Cite this

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title = "IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry",

abstract = "Most MALDI instrumentation uses UV lasers. We have designed a MALDI-IM-oTOF-MS which employs both a Nd:YAG laser pumped optical parametric oscillator OPOTEK, λ = 2.8-3.2 μm at 20 Hz) to perform IR-LDI or IR-MALDI and a Nd:YLF laser (Crystalaser, λ = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined {"}trend lines{"}. Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR-LDI produced similar results as IR-MALDI for the direct tissue analysis of phospholipids from rat brain.",

keywords = "Adducts reduction, Ion-mobility MALDI, IR lasers, UV lasers",

author = "Woods, {Amina S.} and Micheal Ugarov and Jackson, {Shelley N.} and Thomas Egan and Wang, {Hay Yan J} and Murray, {Kermit K.} and Schultz, {J. Albert}",

year = "2006",

month = jun,

doi = "10.1021/pr060055l",

language = "English (US)",

volume = "5",

pages = "1484--1487",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

number = "6",

}

TY - JOUR

T1 - IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry

AU - Woods, Amina S.

AU - Ugarov, Micheal

AU - Jackson, Shelley N.

AU - Egan, Thomas

AU - Wang, Hay Yan J

AU - Murray, Kermit K.

AU - Schultz, J. Albert

PY - 2006/6

Y1 - 2006/6

N2 - Most MALDI instrumentation uses UV lasers. We have designed a MALDI-IM-oTOF-MS which employs both a Nd:YAG laser pumped optical parametric oscillator OPOTEK, λ = 2.8-3.2 μm at 20 Hz) to perform IR-LDI or IR-MALDI and a Nd:YLF laser (Crystalaser, λ = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined "trend lines". Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR-LDI produced similar results as IR-MALDI for the direct tissue analysis of phospholipids from rat brain.

AB - Most MALDI instrumentation uses UV lasers. We have designed a MALDI-IM-oTOF-MS which employs both a Nd:YAG laser pumped optical parametric oscillator OPOTEK, λ = 2.8-3.2 μm at 20 Hz) to perform IR-LDI or IR-MALDI and a Nd:YLF laser (Crystalaser, λ = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined "trend lines". Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR-LDI produced similar results as IR-MALDI for the direct tissue analysis of phospholipids from rat brain.

KW - Adducts reduction

KW - Ion-mobility MALDI

KW - IR lasers

KW - UV lasers

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U2 - 10.1021/pr060055l

DO - 10.1021/pr060055l

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AN - SCOPUS:33744966379

SN - 1535-3893

VL - 5

SP - 1484

EP - 1487

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 6

ER -

IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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