TY - GEN
T1 - Iontophoresis instrumentation for the enhancement of gene therapy in wound healing
AU - Leistner, Martina
AU - Wang, Samantha
AU - Etienne-Cummings, Ralph
AU - Lay, Frank
AU - Born, Louis
AU - Alikhassy, Zahra
AU - Ahmed, Ali Karim
AU - Harmon, John W.
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/9/25
Y1 - 2017/9/25
N2 - Iontophoresis and electroporation are two progressively expanding methods that are used to enhance drug delivery into the human body. They involve the exposure of cells to an external electric field, to drive genetic drugs (plasmid) to a desired location or permeabilize the cell's membranes for the drug to pass. While electroporation has been successful in enhancing plasmid gene therapy in the past, it requires very high local voltages and is less suitable for treating large wounds. Iontophoresis refers to the application of a controlled current at typically lower voltages and is of great interest for clinical wound healing involving naked plasmid transfection. We propose an electrical stimulation setup and instrumentation for meeting the demands of this advancing field. Extensive simulations of electrode placements on rat skin models are performed in COMSOL to determine the most promising setup for in vivo iontophoresis. Electrical circuits for iontophoresis, as well as for the measurement of resulting electrical parameters, are designed, simulated, built and tested. Finally, iontophoresis is performed in vivo (on rat dorsum) using intradermally injected DNA plasmids.
AB - Iontophoresis and electroporation are two progressively expanding methods that are used to enhance drug delivery into the human body. They involve the exposure of cells to an external electric field, to drive genetic drugs (plasmid) to a desired location or permeabilize the cell's membranes for the drug to pass. While electroporation has been successful in enhancing plasmid gene therapy in the past, it requires very high local voltages and is less suitable for treating large wounds. Iontophoresis refers to the application of a controlled current at typically lower voltages and is of great interest for clinical wound healing involving naked plasmid transfection. We propose an electrical stimulation setup and instrumentation for meeting the demands of this advancing field. Extensive simulations of electrode placements on rat skin models are performed in COMSOL to determine the most promising setup for in vivo iontophoresis. Electrical circuits for iontophoresis, as well as for the measurement of resulting electrical parameters, are designed, simulated, built and tested. Finally, iontophoresis is performed in vivo (on rat dorsum) using intradermally injected DNA plasmids.
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U2 - 10.1109/ISCAS.2017.8050729
DO - 10.1109/ISCAS.2017.8050729
M3 - Conference contribution
AN - SCOPUS:85032682519
T3 - Proceedings - IEEE International Symposium on Circuits and Systems
BT - IEEE International Symposium on Circuits and Systems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 50th IEEE International Symposium on Circuits and Systems, ISCAS 2017
Y2 - 28 May 2017 through 31 May 2017
ER -