TY - JOUR
T1 - Involvement of lymphocyte function-associated antigen-1 (LFA-1) but not ICAM-1 in a radioactive leukocyte cell-mediated immunity (LA-CMI) assay.
AU - Fekete, B.
AU - Hildreth, J. E.
AU - Holland, J. F.
AU - Bekesi, J. G.
PY - 1990/3
Y1 - 1990/3
N2 - A number of monoclonal antibodies (Mab) specific to human leukocyte adherence molecules were investigated for their possible function in LA-CMI assay. These data indicate that LFA-1 appears to be directly involved in LA-CMI of human peripheral blood leukocytes since both MAb H52, specific to LFA-1 beta and MAb MHM24, specific to LA-1 alpha inhibited LA-CMI that retained distinct functional characteristics normally exhibited in LFA-1-dependent adhesion steps. That is, binding of cells onto tube wall required the presence of bivalent cations and metabolic energy production; it could be stimulated by phytohemagglutinin (PHA) and was partially dependent on intact cytoskeleton and temperature. MAbs specific to other adherence molecules such as ICAM-1, CD2, CD58, CD4, CD8, CD11b and LAM-1 did not affect leukocyte adherence in this model.
AB - A number of monoclonal antibodies (Mab) specific to human leukocyte adherence molecules were investigated for their possible function in LA-CMI assay. These data indicate that LFA-1 appears to be directly involved in LA-CMI of human peripheral blood leukocytes since both MAb H52, specific to LFA-1 beta and MAb MHM24, specific to LA-1 alpha inhibited LA-CMI that retained distinct functional characteristics normally exhibited in LFA-1-dependent adhesion steps. That is, binding of cells onto tube wall required the presence of bivalent cations and metabolic energy production; it could be stimulated by phytohemagglutinin (PHA) and was partially dependent on intact cytoskeleton and temperature. MAbs specific to other adherence molecules such as ICAM-1, CD2, CD58, CD4, CD8, CD11b and LAM-1 did not affect leukocyte adherence in this model.
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M3 - Article
C2 - 1983768
AN - SCOPUS:0025389240
SN - 0141-2760
VL - 31
SP - 145
EP - 149
JO - Journal of Clinical and Laboratory Immunology
JF - Journal of Clinical and Laboratory Immunology
IS - 3
ER -