TY - JOUR
T1 - Invariant NKT cell lines derived from the NOD·H2h4 mouse enhance autoimmune thyroiditis
AU - Sharma, Rajni B.
AU - Fan, Xiaoguang
AU - Caturegli, Patrizio
AU - Rose, Noel R.
AU - Burek, C. Lynne
PY - 2011
Y1 - 2011
N2 - To study the role of invariant Natural Killer T cell (iNKT) cells in autoimmune thyroiditis, we derived two iNKT cell lines fromthe spleens of NOD· H2h4 mice, a strain that develops spontaneous autoimmune thyroiditis exacerbated by excess dietary iodine. The two lines were CD1d-restricted and expressed CD4+, DX5+, and the Vα4Jα281 gene segment, of the T-cell receptor α locus. Upon stimulation with α-galactosyl-ceramide (α-GalCer), both lines rapidly produced IL-2, IL-4, IFN-γ, IL-10, and TNF-α. Strikingly, a similar cytokine response was also induced by thyroglobulin, one of the most abundant protein in the thyroid gland and a major autoantigen in human autoimmune thyroiditis. Transfer of the iNKT cell lines to syngeneic hosts enhanced autoimmune thyroiditis. Intraperitoneal injections of α-GalCer in iodine primed mice also induced thyroid disease. This paper reports for the first time that iNKT cells respond to thyroglobulin and enhance autoimmune thyroiditis in iodine fed NOD·H2h4 mice.
AB - To study the role of invariant Natural Killer T cell (iNKT) cells in autoimmune thyroiditis, we derived two iNKT cell lines fromthe spleens of NOD· H2h4 mice, a strain that develops spontaneous autoimmune thyroiditis exacerbated by excess dietary iodine. The two lines were CD1d-restricted and expressed CD4+, DX5+, and the Vα4Jα281 gene segment, of the T-cell receptor α locus. Upon stimulation with α-galactosyl-ceramide (α-GalCer), both lines rapidly produced IL-2, IL-4, IFN-γ, IL-10, and TNF-α. Strikingly, a similar cytokine response was also induced by thyroglobulin, one of the most abundant protein in the thyroid gland and a major autoantigen in human autoimmune thyroiditis. Transfer of the iNKT cell lines to syngeneic hosts enhanced autoimmune thyroiditis. Intraperitoneal injections of α-GalCer in iodine primed mice also induced thyroid disease. This paper reports for the first time that iNKT cells respond to thyroglobulin and enhance autoimmune thyroiditis in iodine fed NOD·H2h4 mice.
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U2 - 10.4061/2011/895923
DO - 10.4061/2011/895923
M3 - Article
C2 - 21603172
AN - SCOPUS:84880617465
SN - 2090-8067
VL - 2011
JO - Journal of Thyroid Research
JF - Journal of Thyroid Research
M1 - 895923
ER -