Abstract
This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | Unit 5.12 |
| Journal | Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.] |
| Volume | Chapter 5 |
| DOIs | |
| State | Published - Nov 2001 |
ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
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