Intravitreal plasmin without associated vitrectomy as a treatment for refractory diabetic macular edema

Manuel Diaz-Llopis, Patricia Udaondo, Fernando Arevalo, David Salom, Salvador Garcia-Delpech, Arturo Quijada, Francisco Javier Romero

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Objective: To determine the effectiveness of a low-dose intravitreal injection of autologous plasmin enzyme (APE), without the performance of a vitrectomy, as a treatment for refractory diffuse diabetic macular edema (DDME). Design: Prospective, comparative, interventional case series. Patients: Sixteen patients with bilateral DDME who had not responded to prior laser photocoagulation. All patients received an injection in 1 eye, while the other eye served as a control. Intervention: Intravitreal 0.2 mL APE injection under topical anesthesia. The APE was obtained using a simplified method. Main Outcome Measures: Central macular thickness (CMT) at 1 and 6 months, determined by optical coherence tomography (OCT) and best corrected visual acuity (BCVA). Results: All patients underwent a 1-month follow-up. Prior to injection, CMT in the eye about to receive the injection was 541 ± 79 μm (mean ± standard deviation [SD]) versus 535 ± 76 μm in the control eye. One month after injection, CMT was 241 ± 47 μm in injected eyes and 530 ± 85 μm in control eyes (P < 0.001, bilateral Wilcoxon test for paired samples). The macular edema (ME) improved in all injected eyes (100%), with complete resolution in 7 patients (44%). The mean BCVA of treated eyes was 0.618 ± 0.27 (mean ± SD) at baseline and 0.45 ± 0.24 four weeks after injection (paired samples t-test, P < 0.001). No adverse effects were observed in any of the patients. BCVA and CMT were stable when evaluated at 6-month follow-up (0.43 ± 0.242 and 244 ± 46 μm, respectively). Conclusions: Intravitreal APE injection effectively reduces macular thickening due to DDME in cases that fail to respond to conventional laser photocoagulation, and improves visual acuity in a short term, and this results remain stable in a medium term what is very important. Further investigation is warranted in order to assess long-term efficacy and safety.

Original languageEnglish (US)
Pages (from-to)379-384
Number of pages6
JournalJournal of Ocular Pharmacology and Therapeutics
Issue number4
StatePublished - Aug 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Pharmacology
  • Pharmacology (medical)


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