Internal Antigenic Determinants in Protein Molecules

Teruko Ishizaka; Dan H. Campbell; Kimishige Ishizaka

doi:10.3181/00379727-103-25394

Internal Antigenic Determinants in Protein Molecules

Teruko Ishizaka, Dan H. Campbell, Kimishige Ishizaka

School of Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

1. Positive ring tests and PCA reactions were observed between pepsin-degraded BSA and some of the BSA absorbed anti-BSA rabbit serums, suggesting the presence of the antibody against antigenic determinants which are not on the surface of the native protein molecule. 2. The presence of the antibody to the “hidden sites” did not parallel the concentration of anti-BSA in the serum but rather seemed to be related to the state of the antigen used in the immunization, and to the immunization schedule.

Original language	English (US)
Pages (from-to)	5-9
Number of pages	5
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	103
Issue number	1
DOIs	https://doi.org/10.3181/00379727-103-25394
State	Published - Jan 1960

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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title = "Internal Antigenic Determinants in Protein Molecules",

abstract = "1. Positive ring tests and PCA reactions were observed between pepsin-degraded BSA and some of the BSA absorbed anti-BSA rabbit serums, suggesting the presence of the antibody against antigenic determinants which are not on the surface of the native protein molecule. 2. The presence of the antibody to the “hidden sites” did not parallel the concentration of anti-BSA in the serum but rather seemed to be related to the state of the antigen used in the immunization, and to the immunization schedule.",

author = "Teruko Ishizaka and Campbell, {Dan H.} and Kimishige Ishizaka",

note = "Funding Information: ∗ Ishizaka Teruko † Campbell Dan H. Ishizaka Kimishige ‡ Division of Chemistry and Chemical Engineering, California Inst. of Technology, Pasadena § ∗ The authors wish to thank Dr. W. E. Vannier for advice and interest. This work was supported by a grant from Nat. Inst. of Allergy and Infect. Dis. † Permanent address, Nat. Inst. of Health, Tokyo, Japan. ‡ Fulbright Fellow, premanent address, The Nat. Inst. of Health, Tokyo, Japan. § Contribution No. 2503. 1 1960 103 1 5 9 ",

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AU - Ishizaka, Kimishige

N1 - Funding Information: ∗ Ishizaka Teruko † Campbell Dan H. Ishizaka Kimishige ‡ Division of Chemistry and Chemical Engineering, California Inst. of Technology, Pasadena § ∗ The authors wish to thank Dr. W. E. Vannier for advice and interest. This work was supported by a grant from Nat. Inst. of Allergy and Infect. Dis. † Permanent address, Nat. Inst. of Health, Tokyo, Japan. ‡ Fulbright Fellow, premanent address, The Nat. Inst. of Health, Tokyo, Japan. § Contribution No. 2503. 1 1960 103 1 5 9

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N2 - 1. Positive ring tests and PCA reactions were observed between pepsin-degraded BSA and some of the BSA absorbed anti-BSA rabbit serums, suggesting the presence of the antibody against antigenic determinants which are not on the surface of the native protein molecule. 2. The presence of the antibody to the “hidden sites” did not parallel the concentration of anti-BSA in the serum but rather seemed to be related to the state of the antigen used in the immunization, and to the immunization schedule.

AB - 1. Positive ring tests and PCA reactions were observed between pepsin-degraded BSA and some of the BSA absorbed anti-BSA rabbit serums, suggesting the presence of the antibody against antigenic determinants which are not on the surface of the native protein molecule. 2. The presence of the antibody to the “hidden sites” did not parallel the concentration of anti-BSA in the serum but rather seemed to be related to the state of the antigen used in the immunization, and to the immunization schedule.

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Internal Antigenic Determinants in Protein Molecules

Abstract

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