Interleukin 3-dependent hematopoietic progenitor cell lines

J. S. Greenberger, R. J. Eckner, M. Sakakeeny, P. Marks, D. Reid, G. Nabel, A. Hapel, J. N. Ihle, K. C. Humphries

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


Several biological phenotypes of growth factor-dependent cell lines have been described in recent years, including those with T lymphocyte, neutrophil granulocyte, basophil/mast cell, B lymphocyte, and multipotential stem cell properties. The growth factors for each cell lineage are a subject of intense study. Continuous mouse bone marrow cultures infected with RNA type C viruses (retroviruses) produce nonadherent hematopoietic cells over a longer duration than control cultures. Marrow cultures derived from strains with spontaneously induced ecotropic endogenous retrovirus demonstrate a greater longevity than those from strains with no replicating virus. Cultures infected with murine leukemia virus also generate a greater number, compared with controls, of cloned permanent suspension cell lines dependent for growth on a 41,000-dalton glycoprotein (interleukin 3 [IL 3]). Some are multipotential with capacity for differentiation to erythroid, neutrophil, eosinophil, and basophil/mast cell types. Other cloned IL 3-dependent cell lines are committed to a single pathway. Studies with Friend spleen focus-forming virus indicate that the first effect in the marrow culture is mediated through a subset of adherent hematopoietic stem cells. Bone marrow culture-derived IL 3-dependent cell lines provide a model with which to study the role of viral genes in the control of differentiation and self-renewal capacity of hematopoietic stem cells.

Original languageEnglish (US)
Pages (from-to)2762-2771
Number of pages10
JournalFederation proceedings
Issue number10
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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