Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S. F. Hurst; S. E. Kidd; C. O. Morrissey; E. Snelders; W. J.G. Melchers; M. V. Castelli; E. Mellado; K. Simmon; C. A. Petti; S. Richardson; S. Zhang; A. M. Romanelli; B. L. Wickes; H. A. De Valk; C. H.W. Klaassen; S. Arunmozhi Balajee

doi:10.1128/JCM.00124-09

Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S. F. Hurst, S. E. Kidd, C. O. Morrissey, E. Snelders, W. J.G. Melchers, M. V. Castelli, E. Mellado, K. Simmon, C. A. Petti, S. Richardson, S. Zhang, A. M. Romanelli, B. L. Wickes, H. A. De Valk, C. H.W. Klaassen, S. Arunmozhi Balajee

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

Original language	English (US)
Pages (from-to)	1562-1564
Number of pages	3
Journal	Journal of clinical microbiology
Volume	47
Issue number	5
DOIs	https://doi.org/10.1128/JCM.00124-09
State	Published - May 2009
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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Hurst, S. F., Kidd, S. E., Morrissey, C. O., Snelders, E., Melchers, W. J. G., Castelli, M. V., Mellado, E., Simmon, K., Petti, C. A., Richardson, S., Zhang, S., Romanelli, A. M., Wickes, B. L., De Valk, H. A., Klaassen, C. H. W., & Arunmozhi Balajee, S. (2009). Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus. Journal of clinical microbiology, 47(5), 1562-1564. https://doi.org/10.1128/JCM.00124-09

Hurst, SF, Kidd, SE, Morrissey, CO, Snelders, E, Melchers, WJG, Castelli, MV, Mellado, E, Simmon, K, Petti, CA, Richardson, S, Zhang, S, Romanelli, AM, Wickes, BL, De Valk, HA, Klaassen, CHW & Arunmozhi Balajee, S 2009, 'Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus', Journal of clinical microbiology, vol. 47, no. 5, pp. 1562-1564. https://doi.org/10.1128/JCM.00124-09

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abstract = "Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.",

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AU - Melchers, W. J.G.

AU - Castelli, M. V.

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AU - Richardson, S.

AU - Zhang, S.

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AU - De Valk, H. A.

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Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

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