Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae

Adolfo Saiardi; James J. Caffrey; Solomon H. Snyder; Stephen B. Shears

doi:10.1016/S0014-5793(00)01194-7

Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae

Adolfo Saiardi, James J. Caffrey, Solomon H. Snyder, Stephen B. Shears

School of Medicine

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

The ARGRIII gene of Saccharomyces cerevisiae encodes a transcriptional regulator that also has inositol polyphosphate multikinase (ipmk) activity [Saiardi et al. (1999) Curr. Biol. 9, 1323-1326]. To investigate how inositol phosphates regulate gene expression, we disrupted the ARGRIII gene. This mutation impaired nuclear mRNA export, slowed cell growth, increased cellular [InsP₃] 170-fold and decreased [InsP₆] 100-fold, indicating reduced phosphorylation of InsP₃ to InsP₆. Levels of diphosphoinositol polyphosphates were decreased much less dramatically than was InsP₆. Low levels of InsP₆, and considerable quantities of Ins(1,3,4,5)P₄, were synthesized by an ipmk-independent route. Transcriptional control by ipmk reflects that it is a pivotal regulator of nuclear mRNA export via inositol phosphate metabolism. Copyright (C) 2000 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	28-32
Number of pages	5
Journal	FEBS Letters
Volume	468
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(00)01194-7
State	Published - Feb 18 2000

Keywords

ARGRIII
Inositol phosphate
Inositol polyphosphate multikinase
InsP
Yeast
mRNA export

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(00)01194-7

Cite this

@article{6bf8c1ab7cb4474dbf714bf463b45f27,

title = "Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae",

abstract = "The ARGRIII gene of Saccharomyces cerevisiae encodes a transcriptional regulator that also has inositol polyphosphate multikinase (ipmk) activity [Saiardi et al. (1999) Curr. Biol. 9, 1323-1326]. To investigate how inositol phosphates regulate gene expression, we disrupted the ARGRIII gene. This mutation impaired nuclear mRNA export, slowed cell growth, increased cellular [InsP3] 170-fold and decreased [InsP6] 100-fold, indicating reduced phosphorylation of InsP3 to InsP6. Levels of diphosphoinositol polyphosphates were decreased much less dramatically than was InsP6. Low levels of InsP6, and considerable quantities of Ins(1,3,4,5)P4, were synthesized by an ipmk-independent route. Transcriptional control by ipmk reflects that it is a pivotal regulator of nuclear mRNA export via inositol phosphate metabolism. Copyright (C) 2000 Federation of European Biochemical Societies.",

keywords = "ARGRIII, Inositol phosphate, Inositol polyphosphate multikinase, InsP, Yeast, mRNA export",

author = "Adolfo Saiardi and Caffrey, {James J.} and Snyder, {Solomon H.} and Shears, {Stephen B.}",

note = "Funding Information: We are grateful to Adele M. Snowman for her skillful technical assistance. Supported by USPHS Grant MH18501 (S.H.S.) and Research Scientist Award DA00074 (S.H.S.).",

year = "2000",

month = feb,

day = "18",

doi = "10.1016/S0014-5793(00)01194-7",

language = "English (US)",

volume = "468",

pages = "28--32",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae

AU - Saiardi, Adolfo

AU - Caffrey, James J.

AU - Snyder, Solomon H.

AU - Shears, Stephen B.

N1 - Funding Information: We are grateful to Adele M. Snowman for her skillful technical assistance. Supported by USPHS Grant MH18501 (S.H.S.) and Research Scientist Award DA00074 (S.H.S.).

PY - 2000/2/18

Y1 - 2000/2/18

N2 - The ARGRIII gene of Saccharomyces cerevisiae encodes a transcriptional regulator that also has inositol polyphosphate multikinase (ipmk) activity [Saiardi et al. (1999) Curr. Biol. 9, 1323-1326]. To investigate how inositol phosphates regulate gene expression, we disrupted the ARGRIII gene. This mutation impaired nuclear mRNA export, slowed cell growth, increased cellular [InsP3] 170-fold and decreased [InsP6] 100-fold, indicating reduced phosphorylation of InsP3 to InsP6. Levels of diphosphoinositol polyphosphates were decreased much less dramatically than was InsP6. Low levels of InsP6, and considerable quantities of Ins(1,3,4,5)P4, were synthesized by an ipmk-independent route. Transcriptional control by ipmk reflects that it is a pivotal regulator of nuclear mRNA export via inositol phosphate metabolism. Copyright (C) 2000 Federation of European Biochemical Societies.

AB - The ARGRIII gene of Saccharomyces cerevisiae encodes a transcriptional regulator that also has inositol polyphosphate multikinase (ipmk) activity [Saiardi et al. (1999) Curr. Biol. 9, 1323-1326]. To investigate how inositol phosphates regulate gene expression, we disrupted the ARGRIII gene. This mutation impaired nuclear mRNA export, slowed cell growth, increased cellular [InsP3] 170-fold and decreased [InsP6] 100-fold, indicating reduced phosphorylation of InsP3 to InsP6. Levels of diphosphoinositol polyphosphates were decreased much less dramatically than was InsP6. Low levels of InsP6, and considerable quantities of Ins(1,3,4,5)P4, were synthesized by an ipmk-independent route. Transcriptional control by ipmk reflects that it is a pivotal regulator of nuclear mRNA export via inositol phosphate metabolism. Copyright (C) 2000 Federation of European Biochemical Societies.

KW - ARGRIII

KW - Inositol phosphate

KW - Inositol polyphosphate multikinase

KW - InsP

KW - Yeast

KW - mRNA export

UR - http://www.scopus.com/inward/record.url?scp=0033975636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033975636&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(00)01194-7

DO - 10.1016/S0014-5793(00)01194-7

M3 - Article

C2 - 10683435

AN - SCOPUS:0033975636

SN - 0014-5793

VL - 468

SP - 28

EP - 32

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this