Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae

Adolfo Saiardi, James J. Caffrey, Solomon H. Snyder, Stephen B. Shears

Research output: Contribution to journalArticlepeer-review

118 Scopus citations


The ARGRIII gene of Saccharomyces cerevisiae encodes a transcriptional regulator that also has inositol polyphosphate multikinase (ipmk) activity [Saiardi et al. (1999) Curr. Biol. 9, 1323-1326]. To investigate how inositol phosphates regulate gene expression, we disrupted the ARGRIII gene. This mutation impaired nuclear mRNA export, slowed cell growth, increased cellular [InsP3] 170-fold and decreased [InsP6] 100-fold, indicating reduced phosphorylation of InsP3 to InsP6. Levels of diphosphoinositol polyphosphates were decreased much less dramatically than was InsP6. Low levels of InsP6, and considerable quantities of Ins(1,3,4,5)P4, were synthesized by an ipmk-independent route. Transcriptional control by ipmk reflects that it is a pivotal regulator of nuclear mRNA export via inositol phosphate metabolism. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)28-32
Number of pages5
JournalFEBS Letters
Issue number1
StatePublished - Feb 18 2000


  • Inositol phosphate
  • Inositol polyphosphate multikinase
  • InsP
  • Yeast
  • mRNA export

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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