Inositol hexakisphosphate receptor identified as the clathrin assembly protein AP-2

Susan M. Voglmaier; James H. Keen; Jo Ellen Murphy; Christopher D. Ferris; Glenn D. Prestwich; Solomon H. Snyder; Anne B. Theibert

doi:10.1016/S0006-291X(05)81473-1

Inositol hexakisphosphate receptor identified as the clathrin assembly protein AP-2

Susan M. Voglmaier
, James H. Keen
, Jo Ellen Murphy
, Christopher D. Ferris
, Glenn D. Prestwich
, Solomon H. Snyder
, Anne B. Theibert

School of Medicine

Research output: Contribution to journal › Article › peer-review

114 Scopus citations

Abstract

To clarify the function of the receptor binding protein for inositol hexakisphosphate (IP₆), we obtained a partial amino acid sequence from the purified protein and a partial nucleotide sequence from a cDNA clone of the gene. The sequences are essentially identical to those of the α-subunit of the clathrin assembly protein AP-2. The IP₆ receptor protein analyzed by SDS-PAGE contains a series of subunits which are the same as those of AP-2. Antibodies to AP-2 react with the IP₆ receptor protein in immunoblot analysis.

Original language	English (US)
Pages (from-to)	158-163
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	187
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(05)81473-1
State	Published - Aug 31 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(05)81473-1

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title = "Inositol hexakisphosphate receptor identified as the clathrin assembly protein AP-2",

abstract = "To clarify the function of the receptor binding protein for inositol hexakisphosphate (IP6), we obtained a partial amino acid sequence from the purified protein and a partial nucleotide sequence from a cDNA clone of the gene. The sequences are essentially identical to those of the α-subunit of the clathrin assembly protein AP-2. The IP6 receptor protein analyzed by SDS-PAGE contains a series of subunits which are the same as those of AP-2. Antibodies to AP-2 react with the IP6 receptor protein in immunoblot analysis.",

author = "Voglmaier, \{Susan M.\} and Keen, \{James H.\} and Murphy, \{Jo Ellen\} and Ferris, \{Christopher D.\} and Prestwich, \{Glenn D.\} and Snyder, \{Solomon H.\} and Theibert, \{Anne B.\}",

note = "Funding Information: This work was supported by USPHS grants MH-18501 (S.H.S.), GM-28526 (J.H.K.), NS-29632 (G.D.P.), Research Scientist Award DA-00074 to S.H.S., Training Grant GM-07309 to S.M.V., a grant from the International Life Sciences Institute, a gift from Brlstol-Myers-Squlbb, and a grant from the Lucille P. Markey Foundation for the Neurohlology Research Center to A.B.T. We thank Van Vogel in the Biopolymer Facillty of the Howard Hughes Medical Institute at the Johns Hopkins University School of Medicine for peptlde sequencing. We thank V. Estevez and J. Marecek for synthetic compounds.",

year = "1992",

month = aug,

day = "31",

doi = "10.1016/S0006-291X(05)81473-1",

language = "English (US)",

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pages = "158--163",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Inositol hexakisphosphate receptor identified as the clathrin assembly protein AP-2

AU - Voglmaier, Susan M.

AU - Keen, James H.

AU - Murphy, Jo Ellen

AU - Ferris, Christopher D.

AU - Prestwich, Glenn D.

AU - Snyder, Solomon H.

AU - Theibert, Anne B.

N1 - Funding Information: This work was supported by USPHS grants MH-18501 (S.H.S.), GM-28526 (J.H.K.), NS-29632 (G.D.P.), Research Scientist Award DA-00074 to S.H.S., Training Grant GM-07309 to S.M.V., a grant from the International Life Sciences Institute, a gift from Brlstol-Myers-Squlbb, and a grant from the Lucille P. Markey Foundation for the Neurohlology Research Center to A.B.T. We thank Van Vogel in the Biopolymer Facillty of the Howard Hughes Medical Institute at the Johns Hopkins University School of Medicine for peptlde sequencing. We thank V. Estevez and J. Marecek for synthetic compounds.

PY - 1992/8/31

Y1 - 1992/8/31

N2 - To clarify the function of the receptor binding protein for inositol hexakisphosphate (IP6), we obtained a partial amino acid sequence from the purified protein and a partial nucleotide sequence from a cDNA clone of the gene. The sequences are essentially identical to those of the α-subunit of the clathrin assembly protein AP-2. The IP6 receptor protein analyzed by SDS-PAGE contains a series of subunits which are the same as those of AP-2. Antibodies to AP-2 react with the IP6 receptor protein in immunoblot analysis.

AB - To clarify the function of the receptor binding protein for inositol hexakisphosphate (IP6), we obtained a partial amino acid sequence from the purified protein and a partial nucleotide sequence from a cDNA clone of the gene. The sequences are essentially identical to those of the α-subunit of the clathrin assembly protein AP-2. The IP6 receptor protein analyzed by SDS-PAGE contains a series of subunits which are the same as those of AP-2. Antibodies to AP-2 react with the IP6 receptor protein in immunoblot analysis.

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JO - Biochemical and Biophysical Research Communications

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Inositol hexakisphosphate receptor identified as the clathrin assembly protein AP-2

Abstract

ASJC Scopus subject areas

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